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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Vasconcelos, Mayron Alves de Alves, Ana Cecília Carneiro, Rômulo Farias Dias, Artur Hermano Sampaio Martins, Francisco William Viana Cajazeiras, João Batista Nagano, Celso Shiniti Teixeira, Edson Holanda Nascimento, Kyria Santiago do Cavada, Benildo Sousa |
| Description | Author Affiliation: Vasconcelos MA ( Laboratório Integrado de Biomoléculas-LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160, Fortaleza, Ceará, Brazil. Electronic address: mayronvasconcelos@gmail.com.); Alves AC ( Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.); Carneiro RF ( Laboratório de Biotecnologia Marinha-BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, bloco 871, 60440-970, Fortaleza, Ceará, Brazil.); Dias AH ( Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.); Martins FW ( Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.); Cajazeiras JB ( Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.); Nagano CS ( Laboratório de Biotecnologia Marinha-BioMar-Lab, Departamento de Engenharia de Pesca, Universidade Federal do Ceará, Campus do Pici s/n, bloco 871, 60440-970, Fortaleza, Ceará, Brazil.); Teixeira EH ( Laboratório Integrado de Biomoléculas-LIBS, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, Monsenhor Furtado, s/n, 60430-160, Fortaleza, Ceará, Brazil.); Nascimento KS ( Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil.); Cavada BS ( Laboratório de Moléculas Biologicamente Ativas - BioMol-Lab, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, s/n bloco 907, 60440-970, Fortaleza, Ceará, Brazil. Electronic address: bscavada@gmail.com.) |
| Abstract | This study aimed to purify and characterize a novel mannose-binding lectin from the seeds of Centrolobium microchaete. Centrolobium microchaete lectin (CML) was purified by affinity chromatography in mannose-Sepharose-4B column. CML agglutinated rabbit erythrocytes and was inhibited by D-mannose, -methyl-D-mannoside, D-glucose, N-Acetyl-D-glucosamine and sucrose. The lectin was stable at pH 7.0 and 8.0 and temperatures up to 60°C. The monomeric form of CML showed approximately 28kDa, and its native form is probably a homodimer, as determined by gel filtration chromatography. The primary structure of CML was determined by tandem mass spectrometry that showed CML as a protein with two distinct forms (isolectins CML-1 and CML-2) with 246 and 247 residues, respectively. CML-2 possesses one residue of Asn more than CML-1 in C-terminal. The primary structure of CML agrees with the molecular weights found by electrospray ionization mass spectrometry: 27,224 and 27,338Da for CML-1 and CML-2, respectively. CML is a metal-dependent glycoprotein. Moreover, the glycan composition of CML and its structure were predicted. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 81 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-11-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Fabaceae Chemistry Mannose-binding Lectins Isolation & Purification Seeds Amino Acid Sequence Carbohydrates Hemagglutination Hydrogen-ion Concentration Molecular Sequence Data Molecular Weight Sequence Alignment Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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