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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pereira-Junior, Francisco Nascimento Silva, Helton Colares Freitas, Beatriz Tupinambá Rocha, Bruno Anderson Matias Nascimento, Kyria Santiago Nagano, Celso Shinitti Leal, Rodrigo Bainy Sampaio, Alexandre Holanda Cavada, Benildo Sousa |
| Description | Country affiliation: Brazil Author Affiliation: Pereira-Junior FN ( Department of Biochemistry and Molecular Biology, Federal University of Ceara, Mister Hull s/n Bloco 907, 60.440-970, Fortaleza, CE, Brazil.) |
| Abstract | Platymiscium floribundum lectin (PFL), a mannose/N-acetyl-D-glucosamine-specific lectin, was isolated from P. floribundum seeds using Sepharose-mannose affinity media chromatography. PFL is a glycoprotein that is a potent agglutinin for rabbit erythrocytes. In addition, PFL is highly stable because it is able to maintain its hemagglutinating activity after exposure to temperatures of up to 60 °C for 1 h and exposure to a wide pH range. The PFL purification process was monitored using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the results showed that the purified lectin consists of a single band with a molecular mass of approximately 29 kDa in either the presence or the absence of a reducing agent. The analysis of purified PFL by electrospray ionization-mass spectrometry showed that most ions had a molecular weight of 27,053 ± 2 Da, and other less abundant ions had similar molecular weights. Gel filtration shows that the lectin exists as a dimer in solution with mass at approximately 65 kDa. Sixteen peptides were sequenced, and as a result, a total of 130 amino acids were identified and resulted in a coverage of approximately 65% of the PFL sequence. The partial sequence of PFL was aligned with sequences of other lectins from evolutionarily related species, and PFL showed considerable similarity to the other lectins. |
| File Format | HTM / HTML |
| ISSN | 09523499 |
| Issue Number | 8 |
| Journal | Journal of Molecular Recognition |
| Volume Number | 25 |
| e-ISSN | 10991352 |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2012-08-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Molecular Biology Acetylglucosamine Chemistry Fabaceae Mannose Plant Lectins Isolation & Purification Seeds Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology |
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