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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Luo, Qiu-Lian Tang, Zhuan-Hui Zhang, Xue-Feng Zhong, Yong-Hong Yao, Su-Zhi Wang, Li-Sheng Lin, Cui-Wu Luo, Xuan |
| Description | Author Affiliation: Luo QL ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China.); Tang ZH ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China.); Zhang XF ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China.); Zhong YH ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China.); Yao SZ ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China.); Wang LS ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China); Lin CW ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China); Luo X ( School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, P. R. China) |
| Abstract | In this report, a water-soluble polysaccharide was obtained from the dried stems of Dendrobium officinale Kimura et Migo by hot-water (70-75°C) extraction and 85% ethanol precipitation, and successively purification by DEAE-cellulose anion-exchange chromatography and gel-permeation chromatography. The D. officinale polysaccharide (DOP) has a molecular weight of 8500Da. Monosaccharide composition analysis reveals that DOP is composed of mannose, glucose, and arabinose with a trace of galacturonic acid in a molar ratio of 6.2:2.3:2.1:0.1. Periodate oxidation-smith degradation and 1D and 2D NMR spectroscopy analysis suggest the predominance of mannose and glucose, and it contains a 2-O-acetylglucomannan and (1â4)-linked-ß-d-mannopyranosyl and (1â4)-linked-ß-d-glucopyranosyl residues. Atomic force microscope shows that DOP mainly exists as rod-shaped chains, supporting high degrees of polymerization. The antioxidant activities of the polysaccharide in vitro assay indicate that DOP has good scavenging activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, higher scavenging activity of hydroxyl radical, and metal chelating activities. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Journal | International Journal of Biological Macromolecules |
| Volume Number | 89 |
| e-ISSN | 18790003 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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