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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chen, Guangjing Zhang, Shiqi Ran, Chunxia Wang, Lisha Kan, Jianquan |
| Description | Author Affiliation: Chen G ( College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China); Zhang S ( College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China.); Ran C ( Department of medical technology, Chongqing three gorges medical college, 366 Tianxing Road, Wanzhou, Chongqing 404120, PR China.); Wang L ( College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China.); Kan J ( College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China. Electronic address: kanjianquan@163.com.) |
| Abstract | Single-factor experiment and Box-Behnken design were employed to optimize the ultrasonic-assisted extraction (UAE) of water-soluble polysaccharides from Chinese truffle Tuber huidongense (THWP). The optimal extraction conditions with an extraction yield of 7.17±0.22% crude THWP were determined as follows: ultrasonic power 99.65W, extraction time 40.39min, ratio of water to raw material 24.65mL/g, and extraction temperature 70.1°C. Two purified fractions, THWP-1 and THWP-2 with molecular weights of 128kDa and 729kDa, respectively, were obtained from crude THWP by Cellulose DEAE-52 and Sephadex G-100 chromatography. Monosaccharide component analysis by high performance liquid chromatography (HPLC) indicated that THWP-1 was composed of Glc, Man, and Gal with their corresponding mole percentages of 60.56%, 20.12% and 19.32%, respectively and that THWP-2 contained only Glc. Evaluation of the antioxidant activity in vitro suggested that THWP-1 and THWP-2 had effective scavenging activity of 2,2'-azinobis-(3-ethylbenzothiazolin-6-sulfonicacid) (ABST), hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Accordingly, the polysaccharides from Chinese truffle might serve as a natural antioxidant in functional foods. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Journal | International Journal of Biological Macromolecules |
| Volume Number | 91 |
| e-ISSN | 18790003 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-10-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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