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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pandey, Sanjeev K. Patel, Dinesh K. Maurya, Akhilendra K. Thakur, Ravi Mishra, Durga P. Vinayak, Manjula Haldar, Chandana Maiti, Pralay |
| Description | Author Affiliation: Pandey SK ( Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India. Electronic address: sanjeevbiochem11@gmail.com.); Patel DK ( School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India.); Maurya AK ( Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India.); Thakur R ( Cell Death Research Laboratory, Division of Endocrinology, Central Drug Research Institute, Lucknow 226031, India.); Mishra DP ( Cell Death Research Laboratory, Division of Endocrinology, Central Drug Research Institute, Lucknow 226031, India.); Vinayak M ( Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India.); Haldar C ( Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221 005, India.); Maiti P ( School of Materials Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi 221 005, India. Electronic address: pmaiti.mst@itbhu.ac.in.) |
| Abstract | Tamoxifen (Tmx) embedded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-Tmx) is prepared to evaluate its better DNA cleavage potential, cytotoxicity using Dalton's lymphoma ascite (DLA) cells and MDA-MB231 breast cancer cells. PLGA-Tmx nanoparticles are prepared through emulsified nanoprecipitation technique with varying dimension of 17-30nm by changing the concentrations of polymer, emulsifier and drug. Nanoparticles dimension are measured through electron and atomic force microscopy. Interactions between tamoxifen and PLGA are verified through spectroscopic and calorimetric methods. PLGA-Tmx shows excellent DNA cleavage potential as compared to pure Tmx raising better bioavailability. In vitro cytotoxicity studies indicate that PLGA-Tmx reduces DLA cells viability up to â¼38% against â¼15% in pure Tmx. Hoechst stain is used to detect apoptotic DLA cells through fluorescence imaging of nuclear fragmentation and condensation exhibiting significant increase of apoptosis (70%) in PLGA-Tmx vis-à-vis pure drug (58%). Enhanced DNA cleavage potential, nuclear fragmentation and condensation in apoptotic cells confirm greater bioavailability of PLGA-Tmx as compared to pure Tmx in terms of receptor mediated endocytosis. Hence, the sustained release kinetics of PLGA-Tmx nanoparticles shows much better anticancer efficacy through enhanced DNA cleavage potential and nuclear fragmentation and, thereby, reveal a novel vehicle for the treatment of cancer. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Journal | International Journal of Biological Macromolecules |
| Volume Number | 89 |
| e-ISSN | 18790003 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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