| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Bone, Robert A. Bailey, Charlotte S. L. Wiedermann, Guy Ferjentsik, Zoltan Appleton, Paul L. Murray, Philip J. Maroto, Miguel Dale, J. Kim |
| Description |
Country affiliation: United kingdom Author Affiliation: Bone RA ( Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.); Bailey CS ( Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.); Wiedermann G ( Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.); Ferjentsik Z ( School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, UK.); Appleton PL ( Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.); Murray PJ ( Division of Mathematics, University of Dundee, Dow Street, Dundee DD1 5EH, UK.); Maroto M ( Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.); Dale JK ( Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK j.k.dale@dundee.ac.uk.) |
| Abstract |
During somitogenesis, epithelial somites form from the pre-somitic mesoderm (PSM) in a periodic manner. This periodicity is regulated by a molecular oscillator, known as the 'segmentation clock', that is characterised by an oscillatory pattern of gene expression that sweeps the PSM in a caudal-rostral direction. Key components of the segmentation clock are intracellular components of the Notch, Wnt and FGF pathways, and it is widely accepted that intracellular negative-feedback loops regulate oscillatory gene expression. However, an open question in the field is how intracellular oscillations are coordinated, in the form of spatiotemporal waves of expression, across the PSM. In this study, we provide a potential mechanism for this process. We show at the mRNA level that the Notch1 receptor and Delta-like 1 (Dll1) ligand vary dynamically across the PSM of both chick and mouse. Remarkably, we also demonstrate similar dynamics at the protein level; hence, the pathway components that mediate intercellular coupling themselves exhibit oscillatory dynamics. Moreover, we quantify the dynamic expression patterns of Dll1 and Notch1, and show they are highly correlated with the expression patterns of two known clock components [Lfng mRNA and the activated form of the Notch receptor (cleaved Notch intracellular domain, NICD)]. Lastly, we show that Notch1 is a target of Notch signalling, whereas Dll1 is Wnt regulated. Regulation of Dll1 and Notch1 expression thus links the activity of Wnt and Notch, the two main signalling pathways driving the clock. |
| File Format |
HTM / HTML |
| ISSN |
09501991 |
| e-ISSN |
14779129 |
| DOI |
10.1242/dev.115535 |
| Journal |
Development |
| Issue Number |
24 |
| Volume Number |
141 |
| Language |
English |
| Publisher |
The Company of Biologists |
| Publisher Date |
2014-12-01 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Developmental Discipline Biology Biological Clocks Physiology Gene Expression Regulation, Developmental Intercellular Signaling Peptides And Proteins Metabolism Mesoderm Embryology Receptor, Notch1 Signal Transduction Somites Algorithms Animals Blotting, Western Chick Embryo Glycosyltransferases Image Processing, Computer-assisted Immunohistochemistry In Situ Hybridization In Situ Hybridization, Fluorescence Mice Real-time Polymerase Chain Reaction Receptors, Notch Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Developmental Biology Molecular Biology |
