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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mravec, Jozef Kracun, Stjepan K. Rydahl, Maja G. Westereng, Bjørge Miart, Fabien Clausen, Mads H. Fangel, Jonatan U. Daugaard, Mathilde Van Cutsem, Pierre De Fine Licht, Henrik H. Höfte, Herman Malinovsky, Frederikke G. Domozych, David S. Willats, William G. T. |
| Description | Country affiliation: Denmark Author Affiliation: Mravec J ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.); Kracun SK ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.); Rydahl MG ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.); Westereng B ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas NO-1432, Norway.); Miart F ( Institut Jean-Pierre Bourgin, UMR1318 INRA/AgroParisTech, Saclay Plant Sciences, INRA Centre de Versailles, Versailles 78026, Cedex, France.); Clausen MH ( Center for Nano medicine and Theranostics and Department of Chemistry, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark.); Fangel JU ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.); Daugaard M ( Center for Nano medicine and Theranostics and Department of Chemistry, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark.); Van Cutsem P ( Unité de Recherche en Biologie cellulaire végétale, University of Namur, Namur B-5000, Belgium.); De Fine Licht HH ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.); Höfte H ( Institut Jean-Pierre Bourgin, UMR1318 INRA/AgroParisTech, Saclay Plant Sciences, INRA Centre de Versailles, Versailles 78026, Cedex, France.); Malinovsky FG ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark.); Domozych DS ( Department of Biology and Skidmore Microscopy Imaging Center, Skidmore College, Saratoga Springs, NY 12866, USA.); Willats WG ( Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark willats@plen.ku.dk.) |
| Abstract | Polysaccharides are major components of extracellular matrices and are often extensively modified post-synthetically to suit local requirements and developmental programmes. However, our current understanding of the spatiotemporal dynamics and functional significance of these modifications is limited by a lack of suitable molecular tools. Here, we report the development of a novel non-immunological approach for producing highly selective reciprocal oligosaccharide-based probes for chitosan (the product of chitin deacetylation) and for demethylesterified homogalacturonan. Specific reciprocal binding is mediated by the unique stereochemical arrangement of oppositely charged amino and carboxy groups. Conjugation of oligosaccharides to fluorophores or gold nanoparticles enables direct and rapid imaging of homogalacturonan and chitosan with unprecedented precision in diverse plant, fungal and animal systems. We demonstrated their potential for providing new biological insights by using them to study homogalacturonan processing during Arabidopsis thaliana root cap development and by analyzing sites of chitosan deposition in fungal cell walls and arthropod exoskeletons. |
| File Format | HTM / HTML |
| ISSN | 09501991 |
| e-ISSN | 14779129 |
| Journal | Development |
| Issue Number | 24 |
| Volume Number | 141 |
| Language | English |
| Publisher | The Company of Biologists |
| Publisher Date | 2014-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Developmental Discipline Biology Chitin Metabolism Extracellular Matrix Molecular Probes Oligosaccharides Pectins Arabidopsis Growth & Development Cell Wall Ultrastructure Isolation & Purification Desmidiales Metal Nanoparticles Microarray Analysis Microscopy, Electron, Transmission Molecular Structure Chemistry Optical Imaging Plant Root Cap Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Molecular Biology |
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