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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Slane, Daniel Kong, Jixiang Berendzen, Kenneth W. Kilian, Joachim Henschen, Agnes Kolb, Martina Schmid, Markus Harter, Klaus Mayer, Ulrike De Smet, Ive Bayer, Martin Jürgens, Gerd |
| Description | Country affiliation: Germany Author Affiliation: Slane D ( Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.); Kong J ( Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.); Berendzen KW ( Department of Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.); Kilian J ( Department of Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.); Henschen A ( Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.); Kolb M ( Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.); Schmid M ( Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.); Harter K ( Department of Plant Physiology, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.); Mayer U ( Microscopy facility, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.); De Smet I ( Department of Plant Systems Biology, VIB, Technologiepark 927, Ghent B-9052, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, Ghent B-9052, Belgium Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Boningt); Bayer M ( Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.); Jürgens G ( Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany gerd.juergens@zmbp.uni-tuebingen.de.) |
| Abstract | In multicellular organisms, cellular differences in gene activity are a prerequisite for differentiation and establishment of cell types. In order to study transcriptome profiles, specific cell types have to be isolated from a given tissue or even the whole organism. However, whole-transcriptome analysis of early embryos in flowering plants has been hampered by their size and inaccessibility. Here, we describe the purification of nuclear RNA from early stage Arabidopsis thaliana embryos using fluorescence-activated nuclear sorting (FANS) to generate expression profiles of early stages of the whole embryo, the proembryo and the suspensor. We validated our datasets of differentially expressed candidate genes by promoter-reporter gene fusions and in situ hybridization. Our study revealed that different classes of genes with respect to biological processes and molecular functions are preferentially expressed either in the proembryo or in the suspensor. This method can be used especially for tissues with a limited cell population and inaccessible tissue types. Furthermore, we provide a valuable resource for research on Arabidopsis early embryogenesis. |
| File Format | HTM / HTML |
| ISSN | 09501991 |
| e-ISSN | 14779129 |
| Journal | Development |
| Issue Number | 24 |
| Volume Number | 141 |
| Language | English |
| Publisher | The Company of Biologists |
| Publisher Date | 2014-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Developmental Discipline Biology Arabidopsis Embryology Cell Nucleus Chemistry Gene Expression Profiling Rna, Nuclear Isolation & Purification Metabolism Cloning, Molecular Genotype In Situ Hybridization Microarray Analysis Microscopy, Fluorescence Real-time Polymerase Chain Reaction Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Molecular Biology |
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