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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Vallabani, N. V. Srikanth Karakoti, Ajay S. Singh, Sanjay |
| Description | Country affiliation: India Author Affiliation: Vallabani NV ( Division of Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad 380009, Gujarat, India.); Karakoti AS ( Division of Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad 380009, Gujarat, India); Singh S ( Division of Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Central Campus, Navrangpura, Ahmedabad 380009, Gujarat, India. Electronic address: sanjay.singh@ahduni.edu.in.) |
| Abstract | Fe O nanoparticles (Fe O NPs), demonstrating peroxidase-like activity has garnered attention in the detection of several biomolecules, therefore, emerged as an excellent nano-biosensing agent. The intrinsic peroxidase-like activity of Fe O NPs at acidic pH is the fundamental action driving the oxidation of substrates like TMB, resulting in a colorimetric product formation used in the detection of biomolecules. Hence, the detection sensitivity essentially depends on the ability of oxidation by Fe O NPs in presence of H O . However, the limited sensitivity and pH condition constraint have been identified as the major drawbacks in the detection of biomolecules at physiological pH. Herein, we report overwhelming of the fundamental limitation of acidic pH and tuning the peroxidase-like activity of Fe O NPs at physiological pH by using ATP. In presence of ATP, Fe O NPs exhibited enhanced peroxidase-like activity over a wide range of pH and temperatures. Mechanistically, it was found that the ability of ATP to participate in single electron transfer reaction, through complexation with Fe O NPs, results in the generation of hydroxyl radicals which are responsible for enhanced peroxidase activity at physiological pH. We utilized this ATP-mediated enhanced peroxidase-like activity of Fe O NPs for single step detection of glucose with a colorimetric detection limit of 50µM. Further, we extended this single step detection method to monitor glucose level in human blood serum and detected in a time span of <5min at pH 7.4. |
| File Format | HTM / HTML |
| ISSN | 09277765 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume Number | 153 |
| e-ISSN | 18734367 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-02-09 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Colloid and Surface Chemistry Medicine Physical and Theoretical Chemistry Surfaces and Interfaces Biotechnology |
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