NDLI: A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains

Content Provider	Springer Nature : BioMed Central
Author	Li, Yang Wang, Yumiao Pei, Xiuxiu Chen, Shao Jing, Yang Wu, Yongshuai Ma, Zhiqian Li, Zhiwei Zheng, Zifang Feng, Yingtong Xu, Lele Liu, Xiao Guo, Xuyang Zheng, Haixue Xiao, Shuqi
Abstract	Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant swine viral infectious diseases worldwide. Vaccination is a key strategy for the control and prevention of PRRS. At present, the NADC30-like PRRSV strain has become the predominant epidemic strain in China, superseding the HP-PRRSV strain. The existing commercial vaccines offer substantial protection against HP-PRRSV, but their efficacy against NADC30-like PRRSV is limited. The development of a novel vaccine that can provide valuable cross-protection against both NADC30-like PRRSV and HP-PRRSV is highly important. In this study, an infectious clone of a commercial MLV vaccine strain, GD (HP-PRRSV), was first generated (named rGD). A recombinant chimeric PRRSV strain, rGD-SX-5U2, was subsequently constructed by using rGD as a backbone and embedding several dominant immune genes, including the NSP2, ORF5, ORF6, and ORF7 genes, from an NADC30-like PRRSV isolate. In vitro experiments demonstrated that chimeric PRRSV rGD-SX-5U2 exhibited high tropism for MARC-145 cells, which is of paramount importance in the production of PRRSV vaccines. Moreover, subsequent in vivo inoculation and challenge experiments demonstrated that rGD-SX-5U2 confers cross-protection against both HP-PRRSV and NADC30-like PRRSV, including an improvement in ADG levels and a reduction in viremia and lung tissue lesions. In conclusion, our research demonstrated that the chimeric PRRSV strain rGD-SX-5U2 is a novel approach that can provide broad-spectrum protection against both HP-PRRSV and NADC30-like PRRSV. This may be a significant improvement over previous MLV vaccinations.
Related Links	https://veterinaryresearch.biomedcentral.com/counter/pdf/10.1186/s13567-024-01390-y.pdf
Ending Page	16
Page Count	16
Starting Page	1
File Format	HTM / HTML
ISSN	12979716
DOI	10.1186/s13567-024-01390-y
Journal	Veterinary Research
Issue Number	1
Volume Number	55
Language	English
Publisher	BioMed Central
Publisher Date	2024-10-07
Access Restriction	Open
Subject Keyword	Veterinary Medicine Veterinary Science Virology Microbiology Immunology Porcine reproductive and respiratory syndrome (PRRS) NADC30-like PRRSV HP-PRRSV cross-protection infectious clone genetically engineered vaccine chimeric PRRSV Veterinary Medicine/Veterinary Science
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine
Journal Impact Factor	3.7/2023
5-Year Journal Impact Factor	3.8/2023

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