NDLI: Variation in the prion protein gene (PRNP) open reading frame sequence in French cervids

Content Provider	Springer Nature : BioMed Central
Author	Laubier, Johann Van De Wiele, Anne Barboiron, Aurélie Laloë, Denis Saint-Andrieux, Christine Castille, Johan Meloni, Emma Ernst, Sonja Pellerin, Maryline Floriot, Sandrine Daniel-Carlier, Nathalie Passet, Bruno Merlet, Joël Verheyden, Hélène Béringue, Vincent Andréoletti, Olivier Houston, Fiona Vilotte, Jean-Luc Bourret, Vincent Moazami-Goudarzi, Katayoun
Abstract	The recent emergence of chronic wasting disease (CWD) in Europe has become a new public health risk for monitoring of wild and farmed cervids. This disease, due to prions, has proliferated in North America in a contagious manner. In several mammalian species, polymorphisms in the prion protein gene (PRNP) play a crucial role in the susceptibility to prions and their spread. To obtain a reliable picture of the distribution of PRNP polymorphisms in the two most common cervid species in France, we sequenced the open reading frame (ORF) of this gene in 2114 animals, 1116 roe deer (Capreolus capreolus) and 998 red deer (Cervus elaphus). Selection criteria such as historical origin, spatial distribution and sex ratio have been integrated to establish this sample collection. Except for one heterozygous animal with a non-synonymous mutation at codon 37 (G37A), all the 1116 French roe deer were monomorphic. Red deer showed greater variation with two non-synonymous substitutions (T98A; Q226E), three synonymous substitutions (codons 21, 78 and 136) and a new 24pb deletion (Δ69-77). We found significant regional variations between French regions in the frequency of the identified substitutions. After cloning of the PRNP ORF from animals presenting multiple non-synonymous polymorphisms, we identified six haplotypes and obtained a total of twelve genotypes. As in other European countries, we highlighted the apparent homogeneity of PRNP in the French roe deer and the existence of a greater diversity in the red deer. These results were in line with European phylogeographic studies on these two species.
Related Links	https://veterinaryresearch.biomedcentral.com/counter/pdf/10.1186/s13567-024-01362-2.pdf
Ending Page	14
Page Count	14
Starting Page	1
File Format	HTM / HTML
ISSN	12979716
DOI	10.1186/s13567-024-01362-2
Journal	Veterinary Research
Issue Number	1
Volume Number	55
Language	English
Publisher	BioMed Central
Publisher Date	2024-09-03
Access Restriction	Open
Subject Keyword	Veterinary Medicine Veterinary Science Virology Microbiology Immunology PRNP CWD polymorphism roe deer red deer France Veterinary Medicine/Veterinary Science
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine
Journal Impact Factor	3.7/2023
5-Year Journal Impact Factor	3.8/2023

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