NDLI: Propionibacterium acnes induces cartilaginous endplate degeneration by promoting MIF expression via the NF-κB pathway

Content Provider	Springer Nature : BioMed Central
Author	Zhang, Ying Wang, Yuting Yuan, Yanyan Lin, Yeting Lin, Binbin Zhou, Haiyan
Abstract	Background Propionibacterium acnes (P. acnes) is a novel pathogenic factor that contributes to cartilaginous endplate (CEP) degeneration. However, the underlying mechanism of P. acnes-induced CEP degeneration remains unclear. The objective of this study is to investigate the underlying mechanism of P. acnes-induced CEP degeneration. Methods We first examined MIF expression in degenerated human CEP samples by immunohistochemistry. We developed a P. acnes-induced rat model and detected MIF expression using immunohistochemistry. Additionally, we investigated the mechanism of P. acnes-induced CEP degeneration in CEP cells using western blotting and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results We found that compared with the normal human CEP, the expression of MIF was increased in the degenerated human CEP. In a rat model, P. acnes induced CEP degeneration and upregulated MIF expression significantly. More importantly, we revealed the underlying mechanism of P. acnes-induced CEP degeneration in the rat CEP cells. Firstly, P. acnes induced the expression of MIF in a concentration-dependent manner. Then, MIF upregulated the expression of MMP-13 and promoted the secretion of IL-6 and IL-1β. Finally, P. acnes may promote MIF expression via NF-κB pathway rather than ERK1/2 pathway. Conclusion P. acnes-induced MIF expression via NF-κB pathway may be the underlying mechanism of CEP degeneration.
Related Links	https://josr-online.biomedcentral.com/counter/pdf/10.1186/s13018-020-01714-6.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/s13018-020-01714-6
Journal	Journal of Orthopaedic Surgery and Research
Issue Number	1
Volume Number	15
Language	English
Publisher	BioMed Central
Publisher Date	2020-06-09
Access Restriction	Open
Subject Keyword	Orthopedics Surgical Orthopedics Propionibacterium acnes Cartilaginous endplate degeneration Macrophage migration inhibitory factor NF-κB pathway Inflammatory cytokines
Content Type	Text
Resource Type	Article
Subject	Surgery Orthopedics and Sports Medicine
Journal Impact Factor	2.8/2023
5-Year Journal Impact Factor	3/2023

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