NDLI: In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate

Content Provider	Springer Nature : BioMed Central
Author	Shokouhi Targhi, Hadiseh Mehrbod, Parvaneh Fotouhi, Fatemeh Amininasab, Mehriar
Abstract	Background Influenza A virus (IAV) infection remains a serious public health threat. Due to drug resistance and side effects of the conventional antiviral drugs, repurposing the available natural compounds with high tolerability and fewer side effects has attracted researchers’ attention. The aim of this study was to screen in vitro anti-influenza activity of three anionic compounds ascorbate, acetate, and citrate. Methods The non-cytotoxic concentration of the compounds was determined by MTT assay and examined for the activity against IAV in simultaneous, pre-, and post-penetration combination treatments over 1 h incubation on Madin-Darby Canine Kidney (MDCK) cell line. The virus titer and viral load were determined using hemagglutination assay (HA) and qPCR, respectively. Few pro-inflammatory and anti-inflammatory cytokines were evaluated at RNA and protein levels by qPCR and ELISA, respectively. Results The non-cytotoxic concentrations of the ascorbate (200 mg/ml), acetate and citrate (both 3 mg/ml) reduced the viral titer by 6.5, 4.5, and 1.5 logs in the simultaneous combination treatment. The M protein gene copy number decreased significantly in simultaneous treatment (P < 0.01). The expression of cytokines was also affected by the treatment of these compounds. Conclusions These anionic compounds could affect the influenza virus load, thereby reducing pro-inflammatory cytokines and increasing anti-inflammatory cytokines levels.
Related Links	https://virologyj.biomedcentral.com/counter/pdf/10.1186/s12985-022-01823-0.pdf
Ending Page	12
Page Count	12
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/s12985-022-01823-0
Journal	Virology Journal
Issue Number	1
Volume Number	19
Language	English
Publisher	BioMed Central
Publisher Date	2022-05-23
Access Restriction	Open
Subject Keyword	Virology Ascorbate Acetate Citrate Cytokine Influenza A virus
Content Type	Text
Resource Type	Article
Subject	Virology Infectious Diseases
Journal Impact Factor	4/2023
5-Year Journal Impact Factor	3.8/2023

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate

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Correction to: In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate

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In vitro anti-influenza assessment of anionic compounds ascorbate, acetate and citrate