NDLI: The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization

Content Provider	Springer Nature : BioMed Central
Author	Alqabandi, Maryam de Franceschi, Nicola Maity, Sourav Miguet, Nolwenn Bally, Marta Roos, Wouter H. Weissenhorn, Winfried Bassereau, Patricia Mangenot, Stéphanie
Abstract	Background ESCRT-III proteins are involved in many membrane remodeling processes including multivesicular body biogenesis as first discovered in yeast. In humans, ESCRT-III CHMP2 exists as two isoforms, CHMP2A and CHMP2B, but their physical characteristics have not been compared yet. Results Here, we use a combination of techniques on biomimetic systems and purified proteins to study their affinity and effects on membranes. We establish that CHMP2B binding is enhanced in the presence of PI(4,5)P2 lipids. In contrast, CHMP2A does not display lipid specificity and requires CHMP3 for binding significantly to membranes. On the micrometer scale and at moderate bulk concentrations, CHMP2B forms a reticular structure on membranes whereas CHMP2A (+CHMP3) binds homogeneously. Thus, CHMP2A and CHMP2B unexpectedly induce different mechanical effects to membranes: CHMP2B strongly rigidifies them while CHMP2A (+CHMP3) has no significant effect. Conclusions We therefore conclude that CHMP2B and CHMP2A exhibit different mechanical properties and might thus contribute differently to the diverse ESCRT-III-catalyzed membrane remodeling processes.
Related Links	https://bmcbiol.biomedcentral.com/counter/pdf/10.1186/s12915-021-00983-9.pdf
Ending Page	18
Page Count	18
Starting Page	1
File Format	HTM / HTML
ISSN	17417007
DOI	10.1186/s12915-021-00983-9
Journal	BMC Biology
Issue Number	1
Volume Number	19
Language	English
Publisher	BioMed Central
Publisher Date	2021-04-08
Access Restriction	Open
Subject Keyword	Life Sciences Endosomal sorting complexes Required for Transport (ESCRT) Reconstituted system Bottom up approach Lipid-protein interactions Membrane Mechanical properties Giant unilamellar vesicles (GUV) Micropipette Atomic force microscopy (AFM)
Content Type	Text
Resource Type	Article
Subject	Ecology, Evolution, Behavior and Systematics Structural Biology Biochemistry, Genetics and Molecular Biology Plant Science Biotechnology Physiology Agricultural and Biological Sciences Cell Biology Developmental Biology
Journal Impact Factor	4.4/2023
5-Year Journal Impact Factor	5.4/2023

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