NDLI: Validated method to measure yakuchinone A in plasma by LC-MS/MS and its application to a pharmacokinetic study in rats

Content Provider	Springer Nature : BioMed Central
Author	Chen, Feng Li, Hai-Long Tan, Yin-Feng Guan, Wei-Wei Li, Yong-Hui Zhang, Jun-Qing
Abstract	Background Yakuchinone A has a plethora of beneficial biological effects. However, the pharmacokinetic (PK) data of yakuchinone A still remain unknown so far. Furthermore, the quantification of yakuchinone A in biological samples has not been reported in the literature. Therefore, in the present study we aimed to develop a new method for the fast, efficient and accurate assessment of yakuchinone A concentration in plasma, as a means for facilitating the PK evaluation of yakuchinone A. Results A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the determination of yakuchinone A in rat plasma. Mass spectrometric and chromatographic conditions were optimized. Plasma samples were pretreated by protein precipitation with methanol. LC separation was performed on a Phenomenex Luna C18 column with gradient elution using a mobile phase consisting of methanol–water containing 0.5 mM formic acid (HCOOH) at a flow rate of 0.28 mL/min. ESI-MS spectra were acquired in positive ion multiple reaction monitoring mode (MRM). The precursor-to-product ion pairs used for MRM of yakuchinone A and yakuchinone B were m/z 313.1 → 137.0 and 311.2 → 117.1, respectively. Low concentration of HCOOH reduced the ion suppression caused by matrix components and clearly improved the analytical sensitivity. Yakuchinone A showed good linearity over a wide concentration range (r > 0.99). The accuracy, precision, stability and linearity were found to be within the acceptable criteria. This new method was successfully applied to analyze the rat plasma concentration of parent yakuchinone A after a single oral administration of SuoQuan capsules. Low systemic exposure to parent yakuchinone A was observed. Conclusion The proposed method is sensitive and reliable. It is hoped that this new method will prove useful for the future PK studies.
Related Links	https://bmcchem.biomedcentral.com/counter/pdf/10.1186/1752-153X-8-2.pdf
Ending Page	10
Page Count	10
Starting Page	1
File Format	HTM / HTML
DOI	10.1186/1752-153X-8-2
Journal	BMC Chemistry
Issue Number	1
Volume Number	8
Language	English
Publisher	BioMed Central
Publisher Date	2014-01-14
Access Restriction	Open
Subject Keyword	Chemistry Food Science Yakuchinone A LC-MS/MS Matrix effects HCOOH Pharmacokinetic study SuoQuan capsules Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry
Journal Impact Factor	4.3/2023
5-Year Journal Impact Factor	4.4/2023

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