NDLI: O-Methyltransferase from Soybean Uses Both Caffeoyl-CoA and Flavonoids as Substrates

Content Provider	Springer Nature Link
Author	Kim, Bong Gyu Park, Yong Bae Chong, Youhoon Ahn, Joong Hoon Min, Shin Young Lee, Jung Bok Lee, Yoon Jung Kim, Jong Chan Park, So Hyun Lim, Yoongho Kim, Na Yeon Kim, Dae Hwan
Copyright Year	2003
Abstract	A gene encoding O-methyltransferase (SOMT)-10 from soybean, SOMT-10, was cloned by reverse transcription polymerase chain reaction. Phylogenetic analysis revealed that SOMT-10 belonged to caffeoyl-CoA O-methyltransferase (CCoAOMT) and contained conserved catalytic residues found in CCoAOMT. SOMT-10 was expressed in Escherichia coli as a glutathione S-transferase fusion protein and purified to determine its substrate. Several compounds including caffeoyl-CoA, naringenin, quercetin, caffeic acid, kaempferol and luteonin were tested as substrates for the purified recombinant SOMT-10. Analysis of reaction products using high performance liquid chromatography revealed that SOMT-10 used caffeoyl-CoA, quercetin and luteolin as substrates. This result indicated that SOMT-10 used flavones having vicinal hydroxyl groups. The methylation position was determined to be the 3′ hydroxyl group. It is likely that SOMT-10 is a new class of OMT that uses not only caffeoyl-CoA, but also flavonoids. Molecular docking of tricetin with the modeled structure SOMT-10 disclosed that SOMT-10 showed all combinations of the O-methylated products.
Starting Page	114
Ending Page	120
Page Count	7
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	52
Issue Number	2
e-ISSN	2234344X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	soybean O-methyltransferase Applied Microbiology Biological Techniques caffeoyl-CoA Flavonoid Bioorganic Chemistry Phenylpropanoid
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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