NDLI: Identification of Differential Gene Expression in Juvenile vs. Mature Leaves of Pear (Pyrus pyrifolia) by Using Annealing Control Primer

Content Provider	Springer Nature Link
Author	Kim, Sun Hee Koh, Gab Cheon Lee, Hojoung Hong, Suk Whan Hwang, Baik
Copyright Year	2003
Abstract	The identification of genes specifically expressed at a developmental stage would provide insights into many characteristic features of each of the developmental stages and introduction of molecular markers linked to important agronomic traits. However, the current methods employed to identify genes with specific expression are labor-intensive and suffer from high rates of false positives. Here, we employed a new and accurate reverse transcription-polymerase chain reaction method using annealing control primers (ACP) to identify genes that are preferentially expressed in juvenile or mature leaves of pears (Pyrus pyrifolia L.). We identified and sequenced four of these differentially expressed genes (DEGs). Basic Local Alignment Search Tool searches revealed that one was a hypothetical gene and three genes encoded protein with high similarity to the known genes. These DEGs were further characterized using real-time quantitative PCR to assess their expression pattern. This report suggests that the ACP system is a rapid and accurate method for the identification of development-specific genes in woody plants such as pears, of which a few genes are available.
Starting Page	121
Ending Page	127
Page Count	7
File Format	PDF
ISSN	17382203
Journal	Journal of the Korean Society for Applied Biological Chemistry
Volume Number	52
Issue Number	2
e-ISSN	2234344X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	reverse transcription-polymerase chain reaction developmental specific expression Applied Microbiology differentially expressed genes Biological Techniques phase transition annealing control primers Pyrus pyrifolia Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Biochemistry, Genetics and Molecular Biology

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