NDLI: Antiplatelet activity and isolation of triterpenoidal glycoside from ethanolic extract of Nepeta hindostana

Content Provider	Springer Nature Link
Author	Ansari, Vaseem A. Arif, Muhammad Siddiqui, H. H. Dixit, R. K.
Copyright Year	2016
Abstract	In the present study, triterpenoidal glycoside was isolated from ethanolic extract of aerial part of the plant Nepeta hindostana. The ethanolic extract was subjected to Si-gel CC and compound was isolated. The isolated compound Napedinolside has been characterized as 1β,11α,30-trihydroxy-lup-20(29)ene-(3 → 1′)-β-D-pyranoside on the basis of spectroscopic (UV, IR, $^{1}$ H, $^{13}$ C NMR and MS) methods. The ethanolic and aqueous extract at different concentrations were screened for antiplatelet activity using platelet rich plasma (PRP) prepared by centrifugation of rabbit whole blood (containing 0.9 % sodium citrate as anticoagulant) at 1500 rpm for 15 mins. The Np-01 was identified as triterpenoidal glycoside which was amorphous brownish white powder; yield 0.64 % (w/w); mp 125–127. N. hindostana showed very excellent antiplatelet activity in both aqueous and alcoholic extracts. The minimum concentration showed antiplatelet activity was 0.5 μg/ml. These findings demonstrate that ethanolic and aqueous extract of the selected plant material at different concentrations showed significant antiplatelet activity. The triterpenoidal glycoside from aerial parts of N. hindostana could hold promise for future application in the treatment of blood clot and pulmonary embolism related diseases.
Starting Page	333
Ending Page	337
Page Count	5
File Format	PDF
ISSN	15982386
Journal	Oriental Pharmacy and Experimental Medicine
Volume Number	16
Issue Number	4
e-ISSN	22111069
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-10-04
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Nepeta hindostana Triterpenoidal glycoside Antiplatelet activity Platelet rich plasma Biomedicine Pharmacy
Content Type	Text
Resource Type	Article
Subject	Complementary and Alternative Medicine

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