NDLI: Water hyacinth (Eichhornia crassipes fractions potentially normalize the lead (Pb) poisoning and enhance in vitro thrombolysis

Content Provider	Springer Nature Link
Author	Ahmed, A. M. Abu Khan, Sarowar Jahan Mojumder, Nipun Sharmin, Farjana Rahman, Atiar Bakar, Muhammad Abu Chowdhury, J.M. Kamirul Hasan Azadi, Mohammad Ali
Copyright Year	2016
Abstract	Water hyacinth (Eichhornia crassipes) has been used in phytoextraction to uptake heavy metals and trace elements in various experiments. This research investigated the protective effects of Water hyacinth extracts on Lead (Pb)-induced toxicity in the albino rat. Forty-eight six-weeks-old Wistar albino male rats (average weight, 180 ± 6.45 g) were divided into six groups: normal control (NC), Pb control (PbC), Chloroform extract (ChEx), Ethyl acetate extract (EAEx), Methanol extract (MeEx) and Ascorbic acid treated groups (AA, positive control). All animals except NC group have been administered with Lead acetate (Pb(CH$_{3}$COOH)$_{2}$) before the therapeutic dose. Thrombolytic and cytotoxic effects were evaluated by modified clot lysis and Brine shrimp lethality tests respectively. Biochemical analyses reports revealed that ChEx significantly (P ≤ 0.05) reduced the elevated alkaline phosphatase (ALP) and aspartate amino transferase (AST) whereas MeEx did the alanine amino transferase (ALT) in comparison to positive control. ChEx significantly inhibited the Pb deposition in kidney and liver than two other extracts. ChEx significantly increased the liver protein compared to PbC group. In the thrombolytic assay, EAEx showed the pronounced clot lysis (49.24 %) while ChEx and MeEx showed 45.18 % and 29.13 % of clot lysis respectively. In Brine shrimp lethality bioassay, the LC$_{50}$ value of the ChEx, EAEx and MeEx were 4.16, 4.47 and 9.27 μg/mg respectively and values were statistically significant (p < 0.05) compared to that of reference cytotoxic agent, Vincristine sulfate (LC$_{50’}$ 0.55). Histopathological screening of kidney, liver and spleen showed that ethyl acetate extract recovered the highest of the cellular damage caused by Lead acetate. Biochemical and histopathological screening, therefore, demonstrate that Water hyacinth could be one of the promising sources of normalizing the Pb-poisoning and enhancing the thrombolysis in an animal model. Therapeutic prospects of Water hyacinth could be further studied through highlighting a dose-response study.
Starting Page	321
Ending Page	331
Page Count	11
File Format	PDF
ISSN	15982386
Journal	Oriental Pharmacy and Experimental Medicine
Volume Number	16
Issue Number	4
e-ISSN	22111069
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-10-11
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Eichhornia crassipes Lead poisoning Thrombolysis Cytotoxicity Biomedicine Pharmacy
Content Type	Text
Resource Type	Article
Subject	Complementary and Alternative Medicine

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