NDLI: Pharmacognostic standardization based on physicochemical and molecular parameters of a medicinal mushroom Schizophyllum commune

Content Provider	Springer Nature Link
Author	Acharya, Krishnendu Ghosh, Sandipta Dutta, Arun Kumar
Copyright Year	2016
Abstract	Schizophyllum commune Fr. is valued for its high nutritional composition and medicinal importance. However, quality standards of this species have not been reported till date. The aim of the present study was to provide quality control standardization of S. commune based on organoleptic, microscopic, physico-chemical, chromatographic, molecular features and to partially screen its antioxidant activity. Physico-chemical parameters such as percentage of loss on drying, total ash, swelling capacity, water holding capacity, water solubility index and methanol extractive value were documented. In addition, fluorescence analysis of the powder using 16 different chemical reagents were carried out which exhibit a remarkable variation of colour against treating agents. Quantification of the major bioactive compounds present in the extract showed the following order phenol > flavonoid > ascorbic acid > β-carotene > lycopene. The antioxidant potentials of the extract were determined using experiments of DPPH radical scavenging activity (EC$_{50}$ value 1.07 mg/ml) and total antioxidant capacity (19 μg AAE/mg of extract). Phytochemical screening of the methanolic extract revealed high contents of phenolics which was confirmed by HPLC and HPTLC studies. R$_{f}$ values and their corresponding peaks serve as a signature marker for identification of this mushroom. On the other hand, molecular studies including DNA extraction and sequencing of the Internal Transcribed Spacer region of the genomic DNA serve as a molecular standard. The above distinctive parameters would significantly serve towards establishing the pharmacognostic as well as molecular standards for identification, authentication and quality control of the genuine mushroom.
Starting Page	259
Ending Page	266
Page Count	8
File Format	PDF
ISSN	15982386
Journal	Oriental Pharmacy and Experimental Medicine
Volume Number	16
Issue Number	4
e-ISSN	22111069
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-10-07
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Antioxidant property Edible medicinal mushroom HPLC Molecular analysis Pharmacognostic standardization Physico-chemical evaluation Biomedicine Pharmacy
Content Type	Text
Resource Type	Article
Subject	Complementary and Alternative Medicine

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