NDLI: Induction of apoptosis in human peritoneal mesothelial cells by gastric cancer cell supernatant promotes peritoneal carcinomatosis

Content Provider	Springer Nature Link
Author	Na, Di Song, Yan Jiang, Cheng Gang Sun, Zhe Xu, Ying Ying Wang, Zhen Ning Zhao, Zhuo Zheng Xu, Hui Mian
Copyright Year	2014
Abstract	This study was carried out to evaluate the effects of gastric cancer cell supernatant on human peritoneal mesothelial cell viability and apoptosis and to investigate the mechanism of action of gastric cancer in a mesothelial cell line (HMrSV5). Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (MTT) assay. Mesothelial cells treated with gastric cancer cell supernatant were stained with acridine orange/ethidium bromide and subjected to fluorescence microscopy. C57BL/6 mice were used to establish a cancer invasion model. Morphological changes and exfoliation occurred, and naked areas appeared in both cultured mesothelial cells and the parietal peritoneum after treatment with gastric cancer cell supernatant. Cell supernatant from gastric cancer cells induced apoptosis of mesothelial cells in a time-dependent manner. Obvious morphological changes of cell apoptosis were detected, such as condensation of chromatin, nuclear fragmentations, and apoptotic ladders. These findings demonstrate that gastric cancer cells induce apoptosis of human peritoneal mesothelial cells through supernatants in early peritoneal metastasis.
Starting Page	8301
Ending Page	8307
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	8
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-05-24
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Peritoneal carcinomatosis Stomach neoplasms Mesothelial cell Apoptosis Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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