NDLI: A polysaccharide from pomegranate peels induces the apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway

Content Provider	Springer Nature Link
Author	Li, Jun Zhang, Fujun Wang, Shaohua
Copyright Year	2014
Abstract	Pomegranate (Punica granatum L.) peels, as one of the most valuable by-products of the food industry, has attracted much attention due to its wide range of bioactivities. In this study, the potential anticancer effect of a polysaccharide from pomegranate peels (PPP) on human osteosarcoma cancer cells was investigated. PPP was found to induce the arrest of G2/M phase, induce apoptosis, and inhibit the growth of U-2 osteosarcoma (OS) cells in a dose-dependent manner. Moreover, Western blotting analysis showed that PPP triggered the mitochondrial apoptotic pathway, as indicated by an increase in Bax/Bcl-2 ratios, a loss of mitochondrial membrane potential, release of cytochrome c, activation of caspase-9 and caspase-3, and cleavage of poly(ADP-ribose) polymerase (PARP) in U-2 OS cells. Our results indicate that PPP inhibits the proliferation of human osteosarcoma cancer cells by inducing apoptosis through the intrinsic mitochondrial pathway.
Starting Page	7475
Ending Page	7482
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	8
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-05-02
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Pomegranate (Punica granatum L.) Polysaccharide Osteosarcoma Apoptosis Anticancer activity Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in