NDLI: Activating transcription factor 3 promotes colon cancer metastasis

Content Provider	Springer Nature Link
Author	Wu, Zhi Yong Wei, Zheng mao Sun, Sheng Jie Yuan, Jing Jiao, Shun Chang
Copyright Year	2014
Abstract	Colorectal cancer is one of the commonest of solid malignancy in the world. Activating transcription factor 3 (ATF3), a homolog of the mouse TI-241 and rat LFR-1, is a stress responsive gene that has been widely indicated in different malignancies. However, the role of ATF3 in colon cancer is paradoxical with both a suggested pro- and anti-tumorigenic role. The objective of the current study was to investigate the role of ATF3 in colon cancer metastasis using HT29 and CaCO2 colon cancer cell lines. Expression of ATF3 was initially evaluated in five pairs of colon cancer and matched noncancerous colon tissues. The role of ATF3 in promoting in vitro migration and invasion were evaluated by siRNA-mediated knockdown and adenovirus-mediated overexpression of ATF3. In addition, the role of ATF3 in promoting in vivo tumor growth and hepatic metastasis was investigated by shRNA-mediated knockdown of ATF3. Expression of ATF3 was more in the colon cancer tissues as compared with the pooled noncancerous control colon tissue. Our results showed that in both HT29 and CaCO2 cells, ATF3 promoted in vitro motility and invasion. Furthermore, knockdown of ATF3 attenuated subcutaneous tumor growth and CD31$^{+}$ neovasculature in xenograft assays with HT29 and CaCO2 cells and inhibited hepatic metastasis. Cumulatively, our results unequivocally show that ATF3 promotes colon cancer metastasis.
Starting Page	8329
Ending Page	8334
Page Count	6
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	8
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-05-24
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Colon cancer Activating transcription factor 3 ATF3 HT-29 CaCO2 Metastasis Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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