NDLI: In silico designing, cloning, and heterologous expression of novel chimeric human B lymphocyte CD20 extra loop

Content Provider	Springer Nature Link
Author	Fasihi Ramandi, Mahdi Amani, Jafar Salmanian, Ali Hatef Moazzeni, Seyed Mohammad Ahmadi, Kazem
Copyright Year	2016
Abstract	Design and production of monoclonal antibody for the diagnosis and immunotherapy of non-Hodgkin lymphoma require a suitable CD20 antigen as an effective immunogen. In this study, a new chimeric human CD20 extra loop (hCD20EXL) protein was designed by bioinformatics tools and was expressed in Escherichia coli BL21 DE3. Amino acid sequences, protein structure, immunogenicity, and other physicochemical property of potential antigens were in silico analyzed. Antigenicity, codon optimization, and other predictions of designed protein were determined by bioinformatics tools. The designed protein was heterologously expressed in E. coli and verified by SDS-PAGE and Western blot. Immunogenicity of this antigen was tested in mice, and reactivity of the antibodies was evaluated using flow cytometry. Experimental analysis confirmed the in silico prediction of the designed chimeric hCD20 in this study. Therefore, based on these results, it is suggested that the new chimeric hCD20 antigen could be an appropriate immunogen for production of monoclonal antibody in immunotherapy purposes.
Starting Page	12547
Ending Page	12553
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	9
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-06-27
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Bioinformatics CD20 Heterologous expression Immunotherapy Lymphoma Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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