NDLI: Analysis of APOBEC3A/3B germline deletion polymorphism in breast, cervical and oral cancers from South India and its impact on miRNA regulation

Content Provider	Springer Nature Link
Author	Revathidevi, Sundaramoorthy Manikandan, Mayakannan Rao, Arunagiri Kuha Deva Magendhra Vinothkumar, Vilvanathan Arunkumar, Ganesan Rajkumar, Kottayasamy Seenivasagam Ramani, Rajendran Rajaraman, Ramamurthy Ajay, Chandrasekar Munirajan, Arasambattu Kannan
Copyright Year	2016
Abstract	Breast cancer and cervical cancer are the leading causes of death in women worldwide as well as in India, whilst oral cancer is the top most common cancer among Asian especially in Indian men in terms of both incidence and mortality rate. Genetic factors determining the predisposition to cancer are being explored to identify the signature genetic variations associated with these cancers. Recently, a germline deletion polymorphism in APOBEC3 gene cluster which completely deletes APOBEC3B coding region has been studied for its association with cancer risk. We screened the germline deletion polymorphism in 409 cancer patients (224 breast cancer, 88 cervical cancer and 97 oral cancer samples), 478 controls and 239 cervical cancer tissue DNAs of South Indian origin. The results suggest that the APOBEC3A/3B deletion polymorphism is not significantly associated with cancer risk in our study population (OR 0.739, 95 % CI, p value 0.91457). Considering the viral restriction property of APOBEC3s, we also screened cervical cancer tissue DNAs for the human papilloma virus infection. We observed a gradual increase in the frequency of HPV16 infection from AA/BB cases (66.86 %) to AA/-- cases (71.43) which signifies the impact of this deletion polymorphism in HPV infection. In addition, we performed in silico analysis to understand the effect of this polymorphism on miRNA regulation of the APOBEC3A/3B fusion transcript. Only 8 APOBEC3B targeting miRNAs were observed to regulate the fusion transcript of which miR-34b-3p and miR-138-5p were found to be frequently downregulated in cancers suggesting miRNA-mediated deregulation of APOBEC3A expression in cancer patients harbouring this particular deletion polymorphism.
Starting Page	11983
Ending Page	11990
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	9
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-05-07
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	APOBEC3A APOBEC3B Deletion polymorphism Cancer risk Human Papilloma virus microRNA Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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