NDLI: A polysaccharide from the fruiting bodies of Agaricus blazei Murill induces caspase-dependent apoptosis in human leukemia HL-60 cells

Content Provider	Springer Nature Link
Author	Li, Xiaohui Zhao, Xin Wang, Hongmin Han, Junqing Liu, Li
Copyright Year	2014
Abstract	Polysaccharides are the major active ingredients of fungus Agaricus blazei for treating and preventing cancer. However, there are no reports showing anti-tumor activity of A. blazei polysaccharides (ABP) on human leukemia (HL)-60 cells in vitro and in vivo. In this study, we demonstrated that ABP efficiently inhibited proliferation of cultured HL-60 cells, which was associated with the induction of apoptosis. The increase in ABP-induced apoptosis was accompanied by loss of mitochondria membrane potential (∆Ψm), cytochrome c release from the mitochondria, activation of caspase-3, degradation of poly(ADP-ribose) polymerase (PARP), and the elevated ratio of Bcl-2-associated X (Bax)/B-cell lymphoma 2 (Bcl-2). Moreover, z-DEVD-fmk, a caspase-3 inhibitor, reversed the cytotoxic effects and apoptotic characteristics induced by ABP in HL-60 cells. Furthermore, we confirmed that ABP could obviously inhibit the solid cancer growth of leukemia HL-60 in tumor xenograft model. These data demonstrated that ABP effectively induced the apoptosis of HL-60 cells via a signaling cascade of mitochondrial caspase-3-dependent pathway.
Starting Page	8963
Ending Page	8968
Page Count	6
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	9
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-06
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Agaricus blazei Polysaccharides HL-60 cells Apoptosis Caspase-3 Mitochondria Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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