NDLI: Tumor suppressive microRNA-200a inhibits renal cell carcinoma development by directly targeting TGFB2

Content Provider	Springer Nature Link
Author	Lu, Ruijing Ji, Ziliang Li, Xiaoqing Qin, Jie Cui, Guanghui Chen, Jing Zhai, Qingna Zhao, Chunjuan Zhang, Wei Yu, Zhendong
Copyright Year	2015
Abstract	A large body of evidence indicates that microRNAs play a critical role in tumor initiation and progression by negatively regulating oncogenes or tumor suppressor genes. Here, we report that the expression of miR-200a was notably downregulated in 45 renal cell carcinoma (RCC) samples. Restoration of miR-200a suppressed cell proliferation, migration, and invasion in two RCC cell lines. Furthermore, we used an epithelial-to-mesenchymal transition PCR array to explore the putative target genes of miR-200a. By performing quantitative real-time PCR, ELISA, and luciferase reporter assays, transforming growth factor beta2 (TGFB2) was validated as a direct target gene of miR-200a. Moreover, siRNA-mediated knockdown of TGFB2 partially phenocopied the effect of miR-200a overexpression. These results suggest that miR-200a suppresses RCC development via directly targeting TGFB2, indicating that miR-200a may present a novel target for diagnostic and therapeutic strategies in RCC.
Starting Page	6691
Ending Page	6700
Page Count	10
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	36
Issue Number	9
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-03-27
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	miR-200a Tumor suppressor TGFB2 Renal cell carcinoma Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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