NDLI: Development and characterization of polymorphic microsatellite markers of Fejervarya syhadrensis useful for genetic studies

Content Provider	Springer Nature Link
Author	Aggarwal, Ramesh K. Janani, S. Jegath Sharma, Richa
Copyright Year	2012
Abstract	We describe here ten new microsatellite markers of Fejervarya syhadrensis (long-legged cricket frog). These markers were developed from the short sequence repeat-enriched genomic library and further validated using 34 frog samples collected from different parts of India. All markers were found to be polymorphic revealing 6–30 alleles in the tested samples, with the observed heterozygosity and PIC values varying from 0.067–0.882 to 0.573–0.944, respectively. These markers also showed cross-species/cross-genera transferability to varying extent when tested on four related species of Fejervarya and two species of related genera Hoplobatrachus tigerninus and Euphlyctis cyanophlyctis. Importantly, four markers Fej_ccmb_01, Fej_ccmb_04, Fej_ccmb_06, and Fej_ccmb_07 showed 100 % transferability both across species and genera. Thus, this first report of ten new microsatellite markers is expected to greatly facilitate population genetic studies aimed to understand biogeography of Fejervarya, and/or the effect of various anthropogenic factors on amphibian decline.
Starting Page	799
Ending Page	802
Page Count	4
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	4
Issue Number	3
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-04-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fejervarya Microsatellite markers Conservation Population genetics Cross-species transferability Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology Conservation Biology/Ecology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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