NDLI: Isolation and characterization of eight microsatellite loci from the endangered plant species Hypochaeris salzmanniana (Asteraceae)

Content Provider	Springer Nature Link
Author	Ruas, Claudete F. Nakayama, Thiago J. Ortiz, M. Á. Kuroki, Mayra A. Stuessy, Tod F. Tremetsberger, Karin Ruas, Eduardo A. Oliveira Santos, Melissa Talavera, Salvador Ruas, Paulo M.
Copyright Year	2008
Abstract	We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic information content (PIC) was 0.533 and the observed (H O) and expected (H E) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant deviation from Hardy–Weinberg equilibrium (P ≤ 0.001) and three pairs of loci showed significant linkage disequilibrium (P ≤ 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris.
Starting Page	1413
Ending Page	1416
Page Count	4
File Format	PDF
ISSN	15660621
Journal	Conservation Genetics
Volume Number	10
Issue Number	5
e-ISSN	15729737
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-11-28
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Endangered species Genetic diversity Hypochaeris salzmanniana Microsatellite markers Transferability Plant Sciences Evolutionary Biology Conservation Biology/Ecology Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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