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| Content Provider | Springer Nature Link |
|---|---|
| Author | Valero Jiménez, Claudio A. Pérez Portela, Rocío López Legentil, Susanna |
| Copyright Year | 2011 |
| Abstract | Ten polymorphic microsatellites loci were isolated and characterized from the invasive ascidian Styela plicata by 454 pyrosequencing. From over a hundred potential microsatellite regions, 18 were selected for screening and 10 were retained based on amplification success and marker polymorphism in a sample of 39 individuals from Spain and the United States of America. The number of alleles per locus ranged from 3 to 10, with an average of 6.4 (±2.5 SD). None of the loci showed significant linkage disequilibrium or evidence of large allele drop out or scoring errors. Departure from Hardy–Weinberg equilibrium was observed for two loci in the Spanish population and five loci in the American population, where drastic annual shifts in population genetic structure have been observed. These markers will facilitate further studies on the genetic structure of this species at small spatial and temporal scales. |
| Starting Page | 559 |
| Ending Page | 561 |
| Page Count | 3 |
| File Format | |
| ISSN | 18777252 |
| Journal | Conservation Genetics Resources |
| Volume Number | 4 |
| Issue Number | 3 |
| e-ISSN | 18777260 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2011-12-25 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Sea-squirt Tunicata Pyrosequencing Excess homozygosity Genetic structure Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology Conservation Biology/Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Ecology, Evolution, Behavior and Systematics |
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