NDLI: Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)

Content Provider	Springer Nature Link
Author	Kleven, Oddmund Hallström, Björn M. Hailer, Frank Janke, Axel Hagen, Snorre B. Kopatz, Alexander Eiken, Hans Geir
Copyright Year	2012
Abstract	We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance and interpretability. Applying these assays for the 16 novel microsatellites, we screened 31 brown bears from a northern European population. The number of alleles encountered at single loci was between 4 and 10, and expected and observed heterozygosities ranged between 0.66–0.85 and 0.60–0.97, respectively. Seven of 120 pairwise locus combinations showed linkage disequilibrium and one locus deviated from Hardy–Weinberg equilibrium, while no null-alleles were detected. Our novel multiplex-PCR assays may be useful in forensics, parentage and population genetic studies.
Starting Page	737
Ending Page	741
Page Count	5
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	4
Issue Number	3
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-04-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Di- and tetranucleotide microsatellites High-throughput sequencing Multiplex PCR Non-invasive samples Genetic variation Ursus arctos Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology Conservation Biology/Ecology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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