NDLI: Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties

Please wait, while we are loading the content...

Design, Characterization, and Evaluation of Meloxicam Gel Prepared by Suspension and Solution Polymerization Using Solubility Parameter as the Basis for Development

Tween-Embedded Microemulsions—Physicochemical and Spectroscopic Analysis for Antitubercular Drugs

Ketoprofen Absorption by Muscle and Tendon after Topical or Oral Administration in Patients Undergoing Anterior Cruciate Ligament Reconstruction

Effect of Milling on DSC Thermogram of Excipient Adipic Acid

Inhalation of an Ethanol-Based Zileuton Formulation Provides a Reduction of Pulmonary Adenomas in the A/J Mouse Model

Hydrophobic Thiolation of Pectin with 4-Aminothiophenol: Synthesis and In Vitro Characterization

Design and Formulation of Mebeverine HCl Semisolid Formulations for Intraorally Administration

Valsartan Orodispersible Tablets: Formulation, In vitro/In vivo Characterization

Influence of Microenvironment pH, Humidity, and Temperature on the Stability of Polymorphic and Amorphous Forms of Clopidogrel Bisulfate

Quantitative Determination of Hydrate Content of Theophylline Powder by Chemometric X-ray Powder Diffraction Analysis

Formulation and Release Behavior of Doxycycline–Alginate Hydrogel Microparticles Embedded into Pluronic F127 Thermogels as a Potential New Vehicle for Doxycycline Intradermal Sustained Delivery

Effect of Formulation Parameters on the Preparation of Superporous Hydrogel Self-Nanoemulsifying Drug Delivery System (SNEDDS) of Carvedilol

Effect of Oppositely Charged Polymer and Dissolution Media on Rheology of Spray-Dried Ionic Complexes

The Role of l-arginine in Inclusion Complexes of Omeprazole with Cyclodextrins

Ciprofloxacin as Ocular Liposomal Hydrogel

Stability of 5-Fluoro-2′-deoxycytidine and Tetrahydrouridine in Combination

Effects of Thermal Curing Conditions on Drug Release from Polyvinyl Acetate–Polyvinyl Pyrrolidone Matrices

Stomach-Specific Controlled Release Gellan Beads of Acid-Soluble Drug Prepared by Ionotropic Gelation Method

Preparation and In Vitro Evaluation of a New Fentanyl Patch Based on Functional and Non-functional Pressure Sensitive Adhesives

Composition Optimization and Stability Testing of a Parenteral Antifungal Solution based on a Ternary Solvent System

In Vitro Release Kinetics and Bioavailability of Gastroretentive Cinnarizine Hydrochloride Tablet

Freeze Thaw: A Simple Approach for Prediction of Optimal Cryoprotectant for Freeze Drying

Preparation and Bioavailability Assessment of SMEDDS Containing Valsartan

Dual-Drug Delivery System Based on In Situ Gel-Forming Nanosuspension of Forskolin to Enhance Antiglaucoma Efficacy

Development and Characterization of Enteric-Coated Immediate-Release Pellets of Aceclofenac by Extrusion/Spheronization Technique Using κ-Carrageenan as a Pelletizing Agent

Naked Plasmid DNA Formulation: Effect of Different Disaccharides on Stability after Lyophilisation

Production and Stability Evaluation of Modified-Release Microparticles for the Delivery of Drug Combinations

Formulation and Optimization of Orodispersible Tablets of Diazepam

Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties

Enhancement of Sodium Caprate on Intestine Absorption and Antidiabetic Action of Berberine

Evaluation of Drug Release From Coated Pellets Based on Isomalt, Sugar, and Microcrystalline Cellulose Inert Cores

Chitosan and Glyceryl Monooleate Nanostructures Containing Gemcitabine: Potential Delivery System for Pancreatic Cancer Treatment

Development of a New Method to Get a Reliable Powder Flow Characteristics Using Only 1 to 2 g of Powder

Application of Crustacean Chitin as a Co-diluent in Direct Compression of Tablets

Buccal Drug Delivery of Pravastatin Sodium

The Effects of Rosuvastatin on the Serum Cortisol, Serum Lipid, and Serum Mevalonic Acid Levels in the Healthy Indian Male Population

Modulation of Tramadol Release from a Hydrophobic Matrix: Implications of Formulations and Processing Variables

A Review of Multi-Responsive Membranous Systems for Rate-Modulated Drug Delivery

Evaluation of Chitosan/Alginate Beads Using Experimental Design: Formulation and In Vitro Characterization

Validity of a Power Law Approach to Model Tablet Strength as a Function of Compaction Pressure

Cascade Impactor (CI) Mensuration—An Assessment of the Accuracy and Precision of Commercially Available Optical Measurement Systems

AAPS PharmSciTech : Volume 10

AAPS PharmSciTech : Volume 9

AAPS PharmSciTech : Volume 8

AAPS PharmSciTech : Volume 7

AAPS PharmSciTech : Volume 6

AAPS PharmSciTech : Volume 5

AAPS PharmSciTech : Volume 4

AAPS PharmSciTech : Volume 3

AAPS PharmSciTech : Volume 2

AAPS PharmSciTech : Volume 1

Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties

Content Provider	Springer Nature Link
Author	Sandri, Giuseppina Bonferoni, Maria Cristina Rossi, Silvia Ferrari, Franca Boselli, Cinzia Caramella, Carla
Copyright Year	2010
Abstract	The aim of this paper was to evaluate the penetration enhancement properties of nanoparticles (NP) based on N-trimethyl chitosan (TMC 35% quaternization degree) loaded with insulin. The permeation performances of TMC NP were compared with those of chitosan (CS) NP and also with TMC and CS solutions. To estimate the mechanism of penetration enhancement, two different approaches have been taken into account: an in vitro study (Caco-2 cells) and an ex vivo study (excised rat duodenum, jejunum, and ileum). Insulin-loaded CS and TMC NP had dimensions of about 250 nm and had high yield and high encapsulation efficiency. The in vitro study evidenced that TMC and CS were able to enhance insulin permeation to the same extent. Penetration enhancement properties of TMC NP seem to be prevalently related to endocytosis while the widening of tight junctions appeared more important as mechanism in the case of CS NP. The ex vivo study put in evidence the role of mucus layer and of its microclimate pH. In duodenum (pH 5–5.5), CS and TMC solutions were more effective than NP while TMC NP were more efficient towards jejunum tissue (pH 6–6.5) for their high mucoadhesive potential. Confocal laser scanning microscopy study supported the hypothesis that penetration enhancement due to TMC NP was mainly due to internalization/endocytosis into duodenum and jejunum epithelial cells. The good penetration enhancement properties (permeation and penetration/internalization) make TMC NP suitable carriers for oral administration of insulin.
Starting Page	362
Ending Page	371
Page Count	10
File Format	PDF
Journal	AAPS PharmSciTech
Volume Number	11
Issue Number	1
e-ISSN	15309932
Language	English
Publisher	Springer US
Publisher Date	2010-03-16
Publisher Institution	American Association of Pharmaceutical Scientists
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	absorption properties caco-2 model ex vivo rat intestine model insulin internalization/uptake trimethyl chitosan nanoparticles Pharmacy Biochemistry Biotechnology Pharmacology/Toxicology
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties

Regional permeability of salmon calcitonin in isolated rat gastrointestinal tracts: Transport mechanism using Caco-2 cell monolayer

Uptake and Transport of PEG-Graft-Trimethyl-Chitosan Copolymer–Insulin Nanocomplexes by Epithelial Cells

Influence of charge on FITC-BSA-loaded chondroitin sulfate-chitosan nanoparticles upon cell uptake in human Caco-2 cell monolayers

In vitro Permeability Enhancement in Intestinal Epithelial Cells (Caco-2) Monolayer of Water Soluble Quaternary Ammonium Chitosan Derivatives

Mechanism of Enhanced Oral Absorption of a Nano-Drug Delivery System Loaded with Trimethyl Chitosan Derivatives

Lyophilized insulin nanoparticles prepared from quaternized N-aryl derivatives of chitosan as a new strategy for oral delivery of insulin: in vitro, ex vivo and in vivo characterizations.

Liposomes coated with N-trimethyl chitosan to improve the absorption of harmine in vivo and in vitro

Uptake of Chitosan and Associated Insulin in Caco-2 Cell Monolayers: A Comparison Between Chitosan Molecules and Chitosan Nanoparticles

Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties

Similar Documents

Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties

Regional permeability of salmon calcitonin in isolated rat gastrointestinal tracts: Transport mechanism using Caco-2 cell monolayer

Uptake and Transport of PEG-Graft-Trimethyl-Chitosan Copolymer–Insulin Nanocomplexes by Epithelial Cells

Influence of charge on FITC-BSA-loaded chondroitin sulfate-chitosan nanoparticles upon cell uptake in human Caco-2 cell monolayers

In vitro Permeability Enhancement in Intestinal Epithelial Cells (Caco-2) Monolayer of Water Soluble Quaternary Ammonium Chitosan Derivatives

Mechanism of Enhanced Oral Absorption of a Nano-Drug Delivery System Loaded with Trimethyl Chitosan Derivatives

Lyophilized insulin nanoparticles prepared from quaternized N-aryl derivatives of chitosan as a new strategy for oral delivery of insulin: in vitro, ex vivo and in vivo characterizations.

Liposomes coated with N-trimethyl chitosan to improve the absorption of harmine in vivo and in vitro

Uptake of Chitosan and Associated Insulin in Caco-2 Cell Monolayers: A Comparison Between Chitosan Molecules and Chitosan Nanoparticles

Insulin-Loaded Nanoparticles Based on N-Trimethyl Chitosan: In Vitro (Caco-2 Model) and Ex Vivo (Excised Rat Jejunum, Duodenum, and Ileum) Evaluation of Penetration Enhancement Properties