Loading...
Please wait, while we are loading the content...
Similar Documents
Influence of charge on FITC-BSA-loaded chondroitin sulfate-chitosan nanoparticles upon cell uptake in human Caco-2 cell monolayers
| Content Provider | Scilit |
|---|---|
| Author | Yeh, Ming-Kung Hu, Chieh-Shen Chiang, Chiao-Hsi Hong, Po-Da |
| Copyright Year | 2012 |
| Description | Background and methods: Chondroitin sulfate-chitosan (ChS-CS) nanoparticles and positively and negatively charged fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)-loaded ChS-CS nanoparticles were prepared and characterized. The properties of ChS-CS nanoparticles, including cellular uptake, cytotoxicity, and transepithelial transport, as well as findings on field emission-scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy were evaluated in human epithelial colorectal adenocarcinoma (Caco-2) fibroblasts. ChS-CS nanoparticles with a mean particle size of 250 nm and zeta potentials ranging from –30 to +18 mV were prepared using an ionic gelation method. Results: Standard cell viability assays demonstrated that cells incubated with ChS-CS and FITC-BSA-loaded ChS-CS nanoparticles remained more than 95% viable at particle concentrations up to 0.1 mg/mL. Endocytosis of nanoparticles was confirmed by confocal laser scanning microscopy and measured by flow cytometry. Ex vivo transepithelial transport studies using Caco-2 cells indicated that the nanoparticles were effectively transported into Caco-2 cells via endocytosis. The uptake of positively charged FITC-BSA-loaded ChS-CS nanoparticles across the epithelial membrane was more efficient than that of the negatively charged nanoparticles. Conclusion: The ChS-CS nanoparticles fabricated in this study were effectively endocytosed by Caco-2 fibroblasts without significant cytotoxicity at high nanoparticle concentrations. ChS-CS nanoparticles represent a potential novel delivery system for the transport of hydrophilic macromolecules. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441231/pdf https://www.dovepress.com/getfile.php?fileID=13906 |
| Ending Page | 4872 |
| Page Count | 12 |
| Starting Page | 4861 |
| e-ISSN | 11782013 |
| DOI | 10.2147/IJN.S34770 |
| Journal | International Journal of Nanomedicine |
| Volume Number | 7 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2012-09-01 |
| Access Restriction | Open |
| Subject Keyword | Pharmacology and Pharmacy Nanoparticles Chondroitin Sulfate Chitosan Cell Uptake Cytotoxicity |
| Content Type | Text |
| Resource Type | Article |
