NDLI: Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Content Provider	Springer Nature Link
Author	Opanasopit, Praneet Apirakaramwong, Auayporn Ngawhirunpat, Tanasait Rojanarata, Theerasak Ruktanchai, Uracha
Copyright Year	2007
Abstract	The aim of this study was to investigate the possibility of using pectinate micro/nanoparticles as gene delivery systems. Pectinate micro/nanoparticles were produced by ionotropic gelation. Various factors were studied for their effects on the preparation of pectinate micro/nanoparticles: the pH of the pectin solution, the ratio of pectin to the cation, the concentration of pectin and the cation, and the type of cation (calcium ions, magnesium ions and manganese ions). After the preparation, the size and charge of the pectin micro/nanoparticles and their DNA incorporation efficiency were evaluated. The results showed that the particle sizes decreased with the decreased concentrations of pectin and cation. The type of cations affected the particle size. Sizes of calcium pectinate particles were larger than those of magnesium pectinate and manganese pectinate particles. The DNA loading efficiency showed that Ca-pectinate nanoparticles could entrap DNA up to 0.05 mg when the weight ratio of pectin:CaCl$_{2}$:DNA was 0.2:1:0.05. However, Mg-pectinate could entrap only 0.01 mg DNA when the weight ratio of pectin:MgCl$_{2}$:DNA was 1:100:0.01 The transfection efficiency of both Ca-pectinate and Mg-pectinate nanoparticles yielded relatively low levels of green fluorescent protein expression and low cytotoxicity in Huh7 cells. Given the negligible cytotoxic effects, these pectinate micro/nanoparticles can be considered as potential candidates for use as safe gene delivery carriers.
Starting Page	67
Ending Page	74
Page Count	8
File Format	PDF
Journal	AAPS PharmSciTech
Volume Number	9
Issue Number	1
e-ISSN	15309932
Language	English
Publisher	Springer US
Publisher Date	2008-01-09
Publisher Institution	American Association of Pharmaceutical Scientists
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	gene delivery Huh7 cells micro/nanoparticle pectin Pharmacology/Toxicology Pharmacy Biochemistry Biotechnology
Content Type	Text
Resource Type	Article

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