NDLI: Homogeneous Electrochemical Method for Ochratoxin A Determination Based on Target Triggered Aptamer Hairpin Switch and Exonuclease III-Assisted Recycling Amplification

Content Provider	Springer Nature Link
Author	Liu, Chang Guo, Yajing Luo, Fang Rao, Pingfan Fu, Caili Wang, Shaoyun
Copyright Year	2016
Abstract	A simple, fast, sensitive, and homogeneous electrochemical method has been developed for ochratoxin A (OTA) determination, which combines the advantage of the high selectivity of aptamer to OTA and high efficiency of exonuclease (Exo) III-assisted recycling amplification mechanism. The metastable hairpin probe (HP) was designed into three parts: regions I is the aptamer of OTA as the target recognition; regions II is poly[dA] sequence as extended DNA (E-DNA); regions III as the target DNA (T-DNA) is complementary to a part of the aptamer to stabilize the hairpin-sharp form of the HP in the absence of OTA. The DNA probe with a methylene blue tag at its 3′ terminus (MB-DNA) was designed to be complementary with T-DNA. Introduction of OTA into the assay leads to the formation change of HP from hairpin shape to open form, thus facilitating the hybridization between T-DNA and MB-DNA. The electrochemical signal is amplified through continuous Exo III cleavage. Under the optimal conditions, the differential pulse voltammetric (DPV) response had a linear relationship with the logarithm of OTA concentration in the range of 0.001~0.5 ng mL$^{−1}$. In addition, the developed method has been successfully applied to detect OTA in wheat standard quality control sample. This homogeneous electrochemical sensor may have a potential prospect in detecting other molecules or proteins, which possesses the corresponding aptamer, through easily designing the hairpin probe.
Starting Page	1982
Ending Page	1990
Page Count	9
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	10
Issue Number	6
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2016-12-26
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Homogeneous electrochemical detection Ochratoxin A Recycling amplification Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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