NDLI: Establishment of an Aqueous PEG 200-Based Deep Eutectic Solvent Extraction and Enrichment Method for Pumpkin (Cucurbita moschata) Seed Protein

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Establishment of an Aqueous PEG 200-Based Deep Eutectic Solvent Extraction and Enrichment Method for Pumpkin (Cucurbita moschata) Seed Protein

Content Provider	Springer Nature Link
Author	Liu, Rui Lin Yu, Pei Ge, Xian Li Bai, Xiu Feng Li, Xing Qiang Fu, Qiang
Copyright Year	2016
Abstract	A novel approach for ultrasound–microwave synergistic extraction (UMSE) of pumpkin seed protein was developed using aqueous poly (ethylene glycol) (PEG 200)-based deep eutectic solvent (DES) as a green extraction medium. Key factors controlling the extraction and optimal operating conditions were optimized by combining the one variable at a time and response surface methodology. Results showed that the PEG 200 as a hydrogen bond donor combined with choline chloride as a typical hydrogen bond acceptor had a highest extraction efficiency among different solvents. The optimal extraction parameters were optimized as follows: PEG 200-based DES concentration, 28% w/w; solid to liquid ratio, 28 g mL$^{−1}$; microwave power, 140 W; and extraction temperature, 43 °C. Under the optimal parameters, the actual extraction yield was 93.95 ± 0.23% (n = 3). The precipitation rate of pumpkin seed protein was 97.97% with a precipitation time of only 4 min by using an isoelectric point-ethanol-PEG 200 DES ternary co-precipitation method. Overall, this integrated method of PEG 200-based DES and UMSE exhibits a powerful tool for the rapid and efficient extraction of pumpkin seed protein.
Starting Page	1669
Ending Page	1680
Page Count	12
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	10
Issue Number	6
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2016-11-21
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Deep eutectic solvent Polyethylene glycol Pumpkin seed protein Ultrasound–microwave synergistic extraction Response surface methodology Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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