NDLI: Preparation of Multi-target Fusarium Toxins (Zearalenone, Deoxynivalenol, T-2, and HT-2 Toxins) Immunoaffinity Column Using Polystyrene-Divinylbenzene as Matrix

Content Provider	Springer Nature Link
Author	Zhang, Jianing Ding, Ke Han, Tao Chen, Xiangning
Copyright Year	2016
Abstract	The aim of this study was to establish a purifying immunoaffinity column (IAC) for four kinds of Fusarium toxins (ZEN, DON, T-2, and HT-2 toxins) simultaneously, by using polystyrene-divinylbenzene (PS-DVB) as a column matrix. Four different types of PS-DVB resins were selected to prepare the IACs. Among them, two types of resins, GALAK and Agela, were chosen for IAC preparation. The coupling ratios of the monoclonal antibodies and GALAK or Agela resins were 98.37 and 97.64%, the recoveries of the four Fusarium toxins on the GALAK or Agela IACs were 73.02∼91.97 or 66.16∼112.80%, and the RSDs of recoveries were 2.17∼16.53 or 3.42∼12.25%. They can be applied for the purification of Fusarium toxins from the actual food samples. The GALAK resin was a type of matrix with hydrophilic treatment. The recoveries of four Fusarium toxins on Agela IAC were either too low or too high. The adsorption capacities of the GALAK IAC for the four Fusarium toxins were better than those of the Agela IAC. Hence, the research developed a new method for the preparation of multi-target IAC using PS-DVB resin as matrix of column for determination of Fusarium toxins in food. Then, the GALAK IAC was used for flour samples to detect the feasibility of the application in the actual sample.
Starting Page	2053
Ending Page	2059
Page Count	7
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	10
Issue Number	6
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2016-12-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Polystyrene-divinylbenzene Immunoaffinity columns Fusarium toxins Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Survey on deoxynivalenol, zearalenone, and HT-2/T-2 contamination of cereals and products derived from cereals

Analysis of Aflatoxins, Fumonisins, Deoxynivalenol, Ochratoxin A, Zearalenone, HT-2, and T-2 Toxins in Animal Feed by LC–MS/MS Using Cleanup with a Multi-Antibody Immunoaffinity Column

Determination of Deoxynivalenol and Nivalenol in Wheat by Ultra-Performance Liquid Chromatography/Photodiode-Array Detector and Immunoaffinity Column Cleanup

Simultaneous Determination of Deoxynivalenol, Deoxynivalenol-3-Glucoside and Nivalenol in Wheat Grains by HPLC-PDA with Immunoaffinity Column Cleanup

Optimization and Validation of a Fluorescence Polarization Immunoassay for Rapid Detection of T-2 and HT-2 Toxins in Cereals and Cereal-Based Products

Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study

Determination of Neomycin in Aquatic Products Using an Immunoaffinity Column Coupled to High-Performance Liquid Chromatography

Analytical Procedures for the Determination of Aflatoxin B$_{1}$ in Eggs of Laying Hens Using Immunoaffinity Columns and Liquid Chromatography with Post-Column Derivatisation and Fluorescence Detection

Fusarium toxins in different food samples

Preparation of Multi-target Fusarium Toxins (Zearalenone, Deoxynivalenol, T-2, and HT-2 Toxins) Immunoaffinity Column Using Polystyrene-Divinylbenzene as Matrix

Similar Documents

Survey on deoxynivalenol, zearalenone, and HT-2/T-2 contamination of cereals and products derived from cereals

Analysis of Aflatoxins, Fumonisins, Deoxynivalenol, Ochratoxin A, Zearalenone, HT-2, and T-2 Toxins in Animal Feed by LC–MS/MS Using Cleanup with a Multi-Antibody Immunoaffinity Column

Determination of Deoxynivalenol and Nivalenol in Wheat by Ultra-Performance Liquid Chromatography/Photodiode-Array Detector and Immunoaffinity Column Cleanup

Simultaneous Determination of Deoxynivalenol, Deoxynivalenol-3-Glucoside and Nivalenol in Wheat Grains by HPLC-PDA with Immunoaffinity Column Cleanup

Optimization and Validation of a Fluorescence Polarization Immunoassay for Rapid Detection of T-2 and HT-2 Toxins in Cereals and Cereal-Based Products

Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study

Determination of Neomycin in Aquatic Products Using an Immunoaffinity Column Coupled to High-Performance Liquid Chromatography

Analytical Procedures for the Determination of Aflatoxin B$_{1}$ in Eggs of Laying Hens Using Immunoaffinity Columns and Liquid Chromatography with Post-Column Derivatisation and Fluorescence Detection

Fusarium toxins in different food samples

Preparation of Multi-target Fusarium Toxins (Zearalenone, Deoxynivalenol, T-2, and HT-2 Toxins) Immunoaffinity Column Using Polystyrene-Divinylbenzene as Matrix