NDLI: Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Resonance Immunosensor

Content Provider	Springer Nature Link
Author	Pan, Mingfei Wang, Xiaojun Wang, Junping Lu, Yang Qian, Kun Wang, Shuo
Copyright Year	2016
Abstract	In this research, a reproducible surface plasmon resonance (SPR) immunosensor based on inhibition format was fabricated for stable and sensitive detection of four sulfonamides (SAs) in animal-derived foods. The parameters in the fabrication and measurement process were optimized and discussed in details. The method using the proposed SPR immunosensor was validated and exhibited favorable performance for SAs residues detection in common animal-derived food products, as well as acceptable accuracy (pure milk 88.5–106.2%; egg 89.0–91.5%; chicken muscle 87.2–94.7%; beef 84.8–96.9%; fish 87.8–95.0%), precision (relative standard deviation (n = 3), pure milk 3.2–5.2%; egg 1.1–5.4%; chicken muscle 1.1–5.9%; beef 2.5–7.1%; fish 4.6–5.3%), and sensitivity (IC$_{15}$, pure milk 59.6 ng mL$^{−1}$, egg 56.1 ng mL$^{−1}$, chicken muscle 66.9 ng mL$^{−1}$, beef 63.3 ng mL$^{−1}$, fish 62.7 ng mL$^{−1}$ ). Each detection cycle could finish in less than 5 min, and each SPR chip could reuse 300 analysis cycles with response attenuation of 3.7%. These results have demonstrated that the proposed SPR immunosensor has offered an effective, accurate, sensitive, rapid, and low-cost methodology for SAs residue detection in food sample, and this method has great potential for the routine analysis of large numbers of samples on measuring different kinds of compounds.
Starting Page	2027
Ending Page	2035
Page Count	9
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	10
Issue Number	6
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2016-12-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sulfonamides Surface plasmon resonance Immunosensor Animal-derived foods Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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