NDLI: A Halophilic, Alkalithermostable, Ionic Liquid-Tolerant Cellulase and Its Application in In Situ Saccharification of Rice Straw

Content Provider	Springer Nature Link
Author	Mesbah, ha M. Wiegel, Juergen
Copyright Year	2017
Abstract	A cellulase, En5H, from halophilic, alkalithermophilic Alkalilimnicola sp. NM-DCM1 was expressed and purified. En5H had maximal hydrolytic activity at 55 °C, pH 8.8, and 2.5 M NaCl. En5H is β-1,4 linkage-specific, hydrolyzing carboxymethyl cellulose, Avicel, cellobiose, and p-nitrophenyl β-d-glucopyranoside. En5H was resistant to inhibitors and organic solvents. The half-life of En5H was increased 16–43-fold when incubated in 20% (v/v) of ionic liquids (IL) at 55 °C in the presence of 2.5 M NaCl, and maximal hydrolytic activity of En5H in 10% (v/v) 1-allyl-3-methylimidazolium chloride and 1,3-dimethylimidazolium dimethyl phosphate was 122 and 110%, respectively, as compared with activity in buffer. A cellulase-IL system combining IL pretreatment and enzymatic saccharification was tested. With an enzyme load of 110 U/g rice straw, the conversion of rice straw cellulose and hemicellulose increased by 28% compared with unpretreated rice straw. En5H has potential for use in transformation of lignocellulose to glucose in a single-step process.
Starting Page	583
Ending Page	591
Page Count	9
File Format	PDF
ISSN	19391234
Journal	BioEnergy Research
Volume Number	10
Issue Number	2
e-ISSN	19391242
Language	English
Publisher	Springer US
Publisher Date	2017-03-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cellulase Halophilic Alkalithermophilic Wadi An Natrun Ionic liquid Plant Sciences Plant Genetics & Genomics Plant Breeding/Biotechnology Plant Ecology Wood Science & Technology
Content Type	Text
Resource Type	Article
Subject	Renewable Energy, Sustainability and the Environment Agronomy and Crop Science

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