NDLI: Generation of Octaploid Switchgrass by Seedling Treatment with Mitotic Inhibitors

Content Provider	Springer Nature Link
Author	Yoon, Sangwoong Aucar, Sheyla Hernlem, Bradley J. Edme, Serge Palmer, Nathan Sarath, Gautam Mitchell, Robert Blumwald, Eduardo Tobias, Christian M .
Copyright Year	2016
Abstract	Switchgrass (Panicum virgatum L.) exists as multiple cytotypes with octaploid (8x) and tetraploid (4x) populations occupying distinct, overlapping ranges. These cytotypes tend to show differences in adaptation, yield potential, and other characters, but the specific result of whole-genome duplication is not clear and 8x and 4x switchgrass populations are reproductively isolated with limited genetic exchange. To create new opportunities for population improvement and to study the effects of whole genome duplication on switchgrass, seedling treatment of the tetraploid cultivar Liberty with microtubule inhibitors was used to generate an octaploid population. Resulting octaploids, tetraploids, and cytochimeras were resolved by intercrossing octaploid sectors to produce a population of 19 octaploid families. Fertility of octaploid sectors was significantly reduced relative to tetraploid sectors and caryopsis size significantly increased. Cell size was significantly increased which resulted in quantitative changes to leaf anatomy. During seedling and early vegetative growth stages, no differences in vigor or tillering ability were seen. This technique resulted in efficient genome doubling and was simple to perform. However, aneuploids were also identified with both larger and smaller than expected genome sizes.
Starting Page	344
Ending Page	352
Page Count	9
File Format	PDF
ISSN	19391234
Journal	BioEnergy Research
Volume Number	10
Issue Number	2
e-ISSN	19391242
Language	English
Publisher	Springer US
Publisher Date	2016-10-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Colchicine Bioenergy Polyploidy Cytochimera Tetraploid Autopolyploid Plant Sciences Plant Genetics & Genomics Plant Breeding/Biotechnology Plant Ecology Wood Science & Technology
Content Type	Text
Resource Type	Article
Subject	Renewable Energy, Sustainability and the Environment Agronomy and Crop Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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