NDLI: Microencapsulated multicellular tumor spheroids as a novel in vitro model for drug screening

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Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 4, Issue 2, June 2010

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 4, Issue 1, March 2010

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 3

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 2

Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry : Volume 1

Microencapsulated multicellular tumor spheroids as a novel in vitro model for drug screening

Content Provider	Springer Nature Link
Author	Tsoy, A. M. Zaytseva Zotova, D. S. Edelweiss, E. F. Bartkowiak, A. Goergen, J. L. Vodovozova, E. L. Markvicheva, E. A.
Copyright Year	2010
Abstract	To generate multicellular tumor spheroids (MTS) based on human breast adenocarcinoma MCF-7 cells and to study them as a novel in vitro model for anticancer drug screening, a technique for cell microencapsulation in biocompatible alginate-chitosan microcapsules has been used in this study. Using the MTS based on the MCF-7 cells methotrexate (MTX) cytotoxicity has been investigated. A set of MTS with an average size of 150, 200 and 300 μm was prepared as a function of cultivation time. Cell viability was evaluated after MTS incubation in cultivation medium containing various MTX concentrations (1, 2, 10, 50 and 100 nM) for 48 h. MTS were shown to be markedly more resistant to MTX than the monolayer culture. The increase of the spheroid size was in correlation with the enhanced MTS resistance to MTX. Thus, at 100 nM MTX a number of viable cells in MTS with the size of 300 μm was 2.5-fold higher than that in the monolayer culture. It is suggested that the cells microencapsulated into MTS can better mimic cell behavior in small solid tumors compared to the monolayer culture. In the future MTS could be proposed as a novel in vitro model for anticancer drug screening.
Starting Page	243
Ending Page	250
Page Count	8
File Format	PDF
ISSN	19907508
Journal	Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
Volume Number	4
Issue Number	3
e-ISSN	19907516
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2010-08-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	microencapsulation multicellular tumor spheroids alginate-chitosan microcapsules methotrexate Medicinal Chemistry Bioorganic Chemistry
Content Type	Text
Resource Type	Article
Subject	Biochemistry Clinical Biochemistry Molecular Medicine

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