NDLI: Isomerization of 9c11t/10t12c CLA in Triacylglycerols

Content Provider	Springer Nature Link
Author	Christy, Alfred A.
Copyright Year	2010
Abstract	Isomers of conjugated linoleic acid from 7t9c through 12t14t can be induced by thermal treatment of triacylglycerol samples of 9c11t or 10t12c fatty acids in glass tubes. The formation of conjugated linoleic acids (CLAs) has been observed during thermal induction of the above-mentioned triacylglycerols at 250, 280 and 320°C. The concentrations of isomers formed in the mixture varied depending on the temperature and duration of the heating experiments. The objective of this study was to find a suitable thermal induction temperature and time that can produce most of the isomers of CLAs from the above-mentioned triacylglycerols. Such a mixture would give researchers a reference standard that can be used in the identification of CLAs in GC analyses of relevant samples. Fifteen-microlitre portions of the triacylglycerol samples containing 9c11t/10t12c fatty acid were placed in micro-glass ampoules, sealed under nitrogen and then subjected to thermal treatment. The glass ampoules were removed at regular time intervals, cut open, and part of the samples was analysed by infrared spectroscopy using attenuated internal reflectance technique. The remainder of the samples was subjected to derivatisation into their methyl esters. The methyl esters of the isomerised fatty acids were then analysed by gas chromatography after appropriate dilution in heptane. The results show that the thermally induced glyceride samples of 9c11t/10t12c fatty acids gave CLA profiles containing isomers ranging from 7t9c to 12t14t. However, the concentrations of the isomers are different depending on the duration of the thermal induction. It appears that [1,5] sigmatropic rearrangements and positional isomerisations take place in the heated mixtures. The rearrangements and positional isomerisations are accelerated by increasing temperature. The glyceride samples heated to 325°C form isomers within 30 min and provide a mixture of CLA isomers that can be used as reference sample containing the methyl esters of CLAs.
Starting Page	733
Ending Page	741
Page Count	9
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	45
Issue Number	8
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Thermal induction Isomerisation Conjugated linoleic acids Gas chromatography Microbial Genetics and Genomics Medicinal Chemistry Nutrition Medical Biochemistry Neurochemistry Lipidology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Thermally Induced Isomerisation Kinetics of the 9c11t and 10t12c Conjugated Linoleic Acids in Triacylglycerols as Studied by FT-IR Spectrometry Aided by Gas Chromatography

Conjugated linoleic acids (CLA) as precursors of a distinct family of PUFA

Thermally Induced Isomerization of Trilinolein and Trilinoelaidin at 250 °C: Analysis of Products by Gas Chromatography and Infrared Spectroscopy

Open Access Thermally Induced Isomerisation Kinetics of the 9c11t and 10t12c Conjugated Linoleic Acids in Triacylglycerols as Studied by FT-IR Spectrometry Aided by Gas Chromatography

Impact of Conjugated Linoleic Acid (CLA) on Skeletal Muscle Metabolism

Sources and Bioactive Properties of Conjugated Dietary Fatty Acids

In-Vitro Toxicological and Proteomic Analysis of Furan Fatty Acids Which are Oxidative Metabolites of Conjugated Linoleic Acids

Body Compositional Changes and Growth Alteration in Chicks from Hens Fed Conjugated Linoleic Acid

Sequential Feeding of Lipid Supplement Enriches Beef Adipose Tissues with 18:3n-3 Biohydrogenation Intermediates

Isomerization of 9c11t/10t12c CLA in Triacylglycerols

Similar Documents

Thermally Induced Isomerisation Kinetics of the 9c11t and 10t12c Conjugated Linoleic Acids in Triacylglycerols as Studied by FT-IR Spectrometry Aided by Gas Chromatography

Conjugated linoleic acids (CLA) as precursors of a distinct family of PUFA

Thermally Induced Isomerization of Trilinolein and Trilinoelaidin at 250 °C: Analysis of Products by Gas Chromatography and Infrared Spectroscopy

Open Access Thermally Induced Isomerisation Kinetics of the 9c11t and 10t12c Conjugated Linoleic Acids in Triacylglycerols as Studied by FT-IR Spectrometry Aided by Gas Chromatography

Impact of Conjugated Linoleic Acid (CLA) on Skeletal Muscle Metabolism

Sources and Bioactive Properties of Conjugated Dietary Fatty Acids

In-Vitro Toxicological and Proteomic Analysis of Furan Fatty Acids Which are Oxidative Metabolites of Conjugated Linoleic Acids

Body Compositional Changes and Growth Alteration in Chicks from Hens Fed Conjugated Linoleic Acid

Sequential Feeding of Lipid Supplement Enriches Beef Adipose Tissues with 18:3n-3 Biohydrogenation Intermediates

Isomerization of 9c11t/10t12c CLA in Triacylglycerols