NDLI: Increased Lipid Peroxidation in LDL from Type-2 Diabetic Patients

Content Provider	Springer Nature Link
Author	Colas, Romain Pruneta Deloche, Valérie Guichardant, Michel Luquain Costaz, Céline Cugnet Anceau, Christine Moret, Myriam Vidal, Hubert Moulin, Philippe Lagarde, Michel Calzada, Catherine
Copyright Year	2010
Abstract	Increased oxidative stress is associated with type-2 diabetes and related cardiovascular diseases, but oxidative modification of LDL has been partially characterized. Our aim was to compare the lipid and fatty acid composition as well as the redox status of LDL from diabetic patients and healthy subjects. First, to ensure that isolation of LDL by sequential ultracentrifugation did not result in lipid modifications, lipid composition and peroxide content were determined in LDL isolated either by ultracentrifugation or fast-protein liquid chromatography. Both methods resulted in similar concentrations of lipids, fatty acids, hydroxy-octadecadienoic acid (HODE) and malondialdehyde (MDA). Then, LDLs were isolated by ultracentrifugation from eight type-2 diabetic patients and eight control subjects. Compared to control LDL, diabetic LDL contained decreased cholesteryl esters and increased triglyceride concentrations. Ethanolamine plasmalogens decreased by 49%. Proportions of linoleic acid decreased in all lipid classes, while proportions of arachidonic acid increased in cholesteryl esters. Total HODE concentrations increased by 56%, 12- and 15-hydroxy-eicosatetraenoic acid by 161 and 86%, respectively, and MDA levels increased by twofold. α-Tocopherol concentrations, expressed relative to triglycerides, were lower in LDL from patients compared to controls, while γ-tocopherol did not differ. Overall, LDL from type-2 diabetic patients displayed increased oxidative stress. Determination of hydroxylated fatty acids and ethanolamine plasmalogen depletion could be especially relevant in diabetes.
Starting Page	723
Ending Page	731
Page Count	9
File Format	PDF
ISSN	00244201
Journal	Lipids
Volume Number	45
Issue Number	8
e-ISSN	15589307
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Diabetes Fatty acids Peroxidation Plasmalogens Ultracentrifugation Fast-protein liquid chromatography Microbial Genetics and Genomics Medicinal Chemistry Nutrition Medical Biochemistry Neurochemistry Lipidology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Organic Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Lipids. Author manuscript Page /1 10 Increased lipid peroxidation in LDL from type-2 diabetic patients

Phospholipid Distribution and Phospholipid Fatty Acids of the Tropical Tunicates Eudistoma sp. and Leptoclinides uniorbis

Lipids of the Tail Gland, Body and Muzzle Fur of the Red Fox, Vulpes vulpes

Phospholipid Peroxidation: Lack of Effect of Fatty Acid Pairing

Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Liquid Chromatography–Light Scattering Detector–Mass Spectrometric Analysis of Digested Oxidized Rapeseed Oil

Dietary n-3 HUFA Affects Mitochondrial Fatty Acid β-Oxidation Capacity and Susceptibility to Oxidative Stress in Atlantic Salmon

Sources and Bioactive Properties of Conjugated Dietary Fatty Acids

Identification of Plasmalogen Cardiolipins from Pectinatus by Liquid Chromatography–High Resolution Electrospray Ionization Tandem Mass Spectrometry

Increased Lipid Peroxidation in LDL from Type-2 Diabetic Patients

Similar Documents

Lipids. Author manuscript Page /1 10 Increased lipid peroxidation in LDL from type-2 diabetic patients

Phospholipid Distribution and Phospholipid Fatty Acids of the Tropical Tunicates Eudistoma sp. and Leptoclinides uniorbis

Lipids of the Tail Gland, Body and Muzzle Fur of the Red Fox, Vulpes vulpes

Phospholipid Peroxidation: Lack of Effect of Fatty Acid Pairing

Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Liquid Chromatography–Light Scattering Detector–Mass Spectrometric Analysis of Digested Oxidized Rapeseed Oil

Dietary n-3 HUFA Affects Mitochondrial Fatty Acid β-Oxidation Capacity and Susceptibility to Oxidative Stress in Atlantic Salmon

Sources and Bioactive Properties of Conjugated Dietary Fatty Acids

Identification of Plasmalogen Cardiolipins from Pectinatus by Liquid Chromatography–High Resolution Electrospray Ionization Tandem Mass Spectrometry

Increased Lipid Peroxidation in LDL from Type-2 Diabetic Patients