NDLI: RNAi-mediated resistance against Cotton leaf curl disease in elite Indian cotton (Gossypium hirsutum) cultivar Narasimha

Content Provider	Springer Nature Link
Author	Khatoon, Sameena Kumar, Abhinav Sarin, Neera B. Khan, Jawaid A.
Copyright Year	2016
Abstract	Cotton leaf curl disease (CLCuD) is caused by several distinct begomovirus species in association with disease-specific betasatellite essential for induction of disease symptoms. CLCuD is a serious threat for the cultivation of cotton (Gossypium sp.) and several species in the family Malvaceae. In this study, RNAi-based approach was applied to generate transgenic cotton (Gossypium hirsutum) plants resistant to Cotton leaf curl Rajasthan virus (CLCuRV). An intron hairpin (ihp) RNAi construct capable of expressing dsRNA homologous to the intergenic region (IR) of CLCuRV was designed and developed. Following Agrobacterium tumefaciens-mediated transformation of cotton (G. hirsutum cv. Narasimha) plants with the designed ihpRNAi construct, a total of 9 independent lines of transformed cotton were obtained. The presence of the potential stretch of IR in the transformed cotton was confirmed by PCR coupled with Southern hybridization. Upon inoculation with viruliferous whiteflies, the transgenic plants showed high degree of resistance. None of them displayed any CLCuD symptoms even after 90 days post inoculation. The transformed cotton plants showed the presence of siRNAs. The present study demonstrated that ihp dsRNA-mediated resistance strategy of RNAi is an effective means to combat the CLCuD infection in cotton.
Starting Page	530
Ending Page	537
Page Count	8
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	52
Issue Number	4
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2016-03-30
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cotton leaf curl disease Begomovirus Cotton transformation RNAi Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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AN INSIGHT OF COTTON LEAF CURL VIRUS: A DEVASTATING PLANT PATHOGENIC BEGOMOVIRUS

AN INSIGHT OF COTTON LEAF CURL VIRUS: A DEVASTATING PLANT PATHOGENIC BEGOMOVIRUS

Erratum to: Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton ( Gossypium hirsutum )

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Screening techniques for assessment of resistance in Gossypium hirsutum L. against cotton leaf curl

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Letter to the Editor: Cotton leaf curl Multan betasatellite strains cloned from Gossypium barbadense further supports selection due to host resistance

Introgression of cotton leaf curl disease resistance from Gossypium armourianum to G. hirsutum

RNAi-mediated resistance against Cotton leaf curl disease in elite Indian cotton (Gossypium hirsutum) cultivar Narasimha

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AN INSIGHT OF COTTON LEAF CURL VIRUS: A DEVASTATING PLANT PATHOGENIC BEGOMOVIRUS

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