NDLI: Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

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Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

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Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Content Provider	Springer Nature Link
Author	Hashmi, Jamil A. Zafar, Yusuf Arshad, Muhammad Mansoor, Shahid Asad, Shaheen
Copyright Year	2011
Abstract	Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5′AC1) and C-terminal 783 bp (3′AC1) nucleotides cloned under transcriptional control of the CaMV35S were introduced into cotton (Gossypium hirsutum L.) using LBA4404 strain of Agrobacterium tumefaciens to make use of an interference strategy for impairing cotton leaf curl virus (CLCuV) infection in transgenic cotton. Compared with nontransformed control, we observed that transgenic cotton plants overexpressing either N-terminal (5′AC1) or C-terminal (3′AC1) sequences confer resistance to CLCuV by inhibiting replication of viral genomic and β satellite DNA components. Molecular analysis by Northern blot hybridization revealed high transgene expression in early and late growth stages associated with inhibition of CLCuV replication. Of the eight T$_{1}$ transgenic lines tested, six had delayed and minor symptoms as compared to nontransformed control lines which developed disease symptoms after 2–3 weeks of whitefly-mediated viral delivery. Virus biological assay and growth of T$_{2}$ plants proved that transgenic cotton plants overexpressing 5′- and 3′AC1 displayed high resistance level up to 72, 81%, respectively, as compared to non-transformed control plants following inoculation with viruliferous whiteflies giving significantly high cotton seed yield. Progeny analysis of these plants by polymerase chain reaction (PCR), Southern blotting and virus biological assay showed stable transgene, integration, inheritance and cotton leaf curl disease (CLCuD) resistance in two of the eight transgenic lines having single or two transgene insertions. Transgenic cotton expressing partial AC1 gene of CLCuV can be used as virus resistance source in cotton breeding programs aiming to improve virus resistance in cotton crop.
Starting Page	286
Ending Page	296
Page Count	11
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	42
Issue Number	2
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2011-02-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium CLCuV Cotton transformation Glasshouse T$_{0}$ T$_{1}$ T$_{2}$ NTC Virus resistance Virology Plant Sciences Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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Erratum to: Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Erratum to: Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Studies on epidemiology and molecular diversity of cotton leaf curl virus (CLCuV) in cotton (Gossypium hirsutum L.)

Influence of Different Phosphorus Regimes on Disease Resistance in Two Cotton ( Gossypium Hirsutum L . ) Cultivars Differing in Resistance to Cotton Leaf Curl Virus ( Clcuv )

Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton ( Gossypium hirsutum )

Molecular analysis of virus causing leaf curl disease in cotton (Gossypium hirsutum)

RNAi-mediated resistance against Cotton leaf curl disease in elite Indian cotton (Gossypium hirsutum) cultivar Narasimha

AN INSIGHT OF COTTON LEAF CURL VIRUS: A DEVASTATING PLANT PATHOGENIC BEGOMOVIRUS

Cotton transgenics with Antisense AC1 gene for resistance against cotton leaf curl virus

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

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Erratum to: Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Erratum to: Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences

Studies on epidemiology and molecular diversity of cotton leaf curl virus (CLCuV) in cotton (Gossypium hirsutum L.)

Influence of Different Phosphorus Regimes on Disease Resistance in Two Cotton ( Gossypium Hirsutum L . ) Cultivars Differing in Resistance to Cotton Leaf Curl Virus ( Clcuv )

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AN INSIGHT OF COTTON LEAF CURL VIRUS: A DEVASTATING PLANT PATHOGENIC BEGOMOVIRUS

Cotton transgenics with Antisense AC1 gene for resistance against cotton leaf curl virus

Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences