NDLI: Comparative sequence analysis of full-length genome of FIPV at different tissue passage levels

Content Provider	Springer Nature Link
Author	Phillips, J. E. Hilt, D. A. Jackwood, M. W.
Copyright Year	2013
Abstract	Feline infectious peritonitis virus (FIPV), an alpha Coronavirus, is the causative agent of a fatal immune mediated disease in cats. It is currently unclear if this virus circulates in the field or develops in felines that are infected with Feline enteric coronavirus. To better understand the genomic changes associated with viral adaptation, we sequenced the complete genomes of FIPV WSU 79-1146 at different tissue passage levels: passage 1, passage 8, and passage 50 tissue culture. Twenty-one amino acid differences were observed in the polyprotein 1a/ab between the different passages. Only one residue change was observed in the spike glycoprotein, which reverted back on subsequent passages, four changes were observed in the 3c protein, and one change was observed in each 3a, small membrane, nucleocapsid and 7a proteins. The mutation rate was calculated to be 5.08–6.3 × 10$^{−6}$ nucleotides/site/passage in tissue culture suggesting a relatively stable virus. Our data show that FIPV has a low mutation rate as it is passed in cell culture but has the capacity for change specifically in nsp 2, 3c, and 7b as it is passed in cell culture.
Starting Page	490
Ending Page	497
Page Count	8
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	47
Issue Number	3
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2013-08-31
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Feline infectious peritonitis virus Feline enteric coronavirus Pathogenicity Adaptation Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

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