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| Content Provider | Springer Nature Link |
|---|---|
| Author | Oh, Sung Kim, Seongdae Vid, Nagarajan Koo, Jung Mo Jang, Kyung Min Choi, Chang Won Kim, Seong Hwan Kim, Young Shik |
| Copyright Year | 2013 |
| Abstract | A polymerase chain reaction (PCR) using two sets of primers designed from published Tomato yellow leaf curl virus (TYLCV) genomes was developed to distinguish from the TYLCV-IL groups. The specificity of the two sets of primers was proven by testing against control TYLCV genomes and the symptomatic leaves of 34 different tomato cultivars naturally infected with TYLCV in greenhouses. One set for TYLCV-IL strain-specific primers (TYLCV-UNI-F and TYLCV-UNI-R) amplified full-length genome fragments from all the 34 tomato cultivars. Another set for TYLCV-IL group-II strain-specific primers (TYLCV-GPII-F and TYLCV-GPII-R) amplified target DNA fragments from only 9 tomato cultivars. Digestion by BglII and EcoRV of the PCR amplicons produced restriction fragment length polymorphism pattern that distinguished the TYLCV-IL group-I with two fragments from the TYLCV-IL group-II with no digested fragment. PCR coupled with BglII and EcoRV digestion confirmed that the 9 tomato cultivars were infected with the TYLCV-IL group-II and the remained 25 tomato cultivars were infected with the TYLCV-IL group-I. |
| Starting Page | 579 |
| Ending Page | 583 |
| Page Count | 5 |
| File Format | |
| ISSN | 09208569 |
| Journal | Virus Genes |
| Volume Number | 47 |
| Issue Number | 3 |
| e-ISSN | 1572994X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2013-07-25 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | TYLCV-IL genetic group-I and group-II PCR–RFLP Tomato yellow leaf curl virus (TYLCV) Tomato cultivars Medical Microbiology Virology Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology Genetics Medicine Molecular Biology |
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