NDLI: Hepatitis B virus subgenotype A1, occurrence of subgenotype D4, and S gene mutations among voluntary blood donors in Kenya

Content Provider	Springer Nature Link
Author	Kwange, Simeon Owuor Budambula, Nancy L. M. Kiptoo, Michael Kibet Okoth, Fredrick Ochwoto, Missiani Oduor, Margaret Kimotho, James Hungo
Copyright Year	2013
Abstract	Kenya is one of the high endemic zones for hepatitis B virus (HBV) infection. The consensuses on prevalence of the HBV genotypes and the existence of their variants have not been fully established in Kenya. Hence, there is a need to further monitor the diversity of HBV. This study aimed to extend the current molecular and epidemiological information about the geographical distribution of HBV genotypes and subgenotypes, as well as to describe the hepatitis B surface antigen (HBsAg) variants circulating in different Regional Blood Transfusion Centres of Kenya. A total of 32 HBsAg positive blood units from five different blood transfusion centers in Kenya were used in the study. The HBV DNA preS/S-gene was amplified and sequenced. Alignments of S gene were applied using reference sequence from GeneBank. Phylogenetic analysis was performed using the MEGAv4.0 software with the neighbor-joining and maximum composite likelihood methods. Twenty-one plasma samples (65.6 %) were DNA positive and were successfully sequenced. Eighteen out of the twenty-one isolates (85.7 %) belonged to subgenotype A1 Afro-Asian: six were from Nairobi, four from Kisumu, two from Embu, and three each from Eldoret and Mombasa. The other three strains (14.3 %, 3/21) belonged to subgenotype D4 from Mombasa. The HBsAg mutations were detected in nine isolates (42.9 %, 9/21). The HBV/A1 and HBV/D4 are dominant among blood donors in Kenya. This demonstrates that continuous monitoring of the HBV diversity would help reveal circulating genotypes and subgenotypes as well as mutants of clinical significance in Kenya.
Starting Page	448
Ending Page	455
Page Count	8
File Format	PDF
ISSN	09208569
Journal	Virus Genes
Volume Number	47
Issue Number	3
e-ISSN	1572994X
Language	English
Publisher	Springer US
Publisher Date	2013-09-05
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hepatitis B virus Hepatitis B surface antigen Mutation Subgenotype Kenya Medical Microbiology Virology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Virology Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China

First hepatitis B virus full-length genomic study among blood donors from Argentina: unexpected mutations in the circulating subgenotypes’ proteins

Mutations in the S gene region of hepatitis B virus genotype D in Golestan Province-Iran

Mutations in the S gene region of hepatitis B virus genotype D in Turkish patients

E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus

Prevalence of occult hepatitis B virus infection among the blood donors in Golestan province: cross-sectional study

Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines

Low seroprevalence of hepatitis delta virus co-infection in hepatitis B virus-infected blood donors in China: A multicenter study

Low Risk of Occult Hepatitis B Infection among Vietnamese Blood Donors.

Hepatitis B virus subgenotype A1, occurrence of subgenotype D4, and S gene mutations among voluntary blood donors in Kenya

Similar Documents

Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China

First hepatitis B virus full-length genomic study among blood donors from Argentina: unexpected mutations in the circulating subgenotypes’ proteins

Mutations in the S gene region of hepatitis B virus genotype D in Golestan Province-Iran

Mutations in the S gene region of hepatitis B virus genotype D in Turkish patients

E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus

Prevalence of occult hepatitis B virus infection among the blood donors in Golestan province: cross-sectional study

Novel quasi-subgenotype D2 of hepatitis B virus identified in Taiwanese aborigines

Low seroprevalence of hepatitis delta virus co-infection in hepatitis B virus-infected blood donors in China: A multicenter study

Low Risk of Occult Hepatitis B Infection among Vietnamese Blood Donors.

Hepatitis B virus subgenotype A1, occurrence of subgenotype D4, and S gene mutations among voluntary blood donors in Kenya