NDLI: Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores

Content Provider	Springer Nature Link
Author	Sinha, Rakesh Kumar Eudes, François
Copyright Year	2015
Abstract	Functional damage of mitochondria and chloroplasts under stress contributes to reactive oxygen species (ROS) induced cell death. A high percentage of cell mortality during microspore culture is a limiting factor in haploid and doubled haploid plant production. In the present study, we studied the contribution of dimethyl tyrosine (DMT) conjugated short peptides to microspore embryogenesis. These DMT-peptides, which are known to translocate to subcellular targets and scavenge ROS, mitigate the effect oxidative stress plays on microspore viability and embryogenesis. The number of viable microspores was significantly higher in the presence of SS-31 and caspase-3-inhibitor (Ac-DEVD-CHO). In particular, the total number of green plant regeneration was increased by 42 % in the presence of SS-02, and by 55 % in the presence of SS-31, in triticale. Conversely, lower caspase-3-like activities were observed in the presence of SS-31 and Ac-DEVD-CHO, and intracellular ROS was reduced in the presence of SS-31, supporting the involvement of SS-31 in reducing microspore cell death by mitigating ROS and caspase-3-like activity. This study further supports the concept that antioxidant conjugated peptides offer a useful strategy for reducing ROS in plant cells.
Starting Page	227
Ending Page	237
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	122
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-04-04
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oxidative stress Cell death Microspore Cell penetrating peptides Microspore embryogenesis Dimethyl tyrosine Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat

Ethylene inhibitors improve efficiency of microspore embryogenesis in hexaploid triticale

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Induction medium osmolality improves microspore embryogenesis in wheat and triticale

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.)

Translocation of cell-penetrating peptides and delivery of their cargoes in triticale microspores

Organelle antioxidants improve microspore embryogenesis in wheat and triticale

DsDNA and protein co-delivery in triticale microspores

Gametic embryogenesis through isolated microspore culture in Corylus avellana L.

Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores

Similar Documents

Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat

Ethylene inhibitors improve efficiency of microspore embryogenesis in hexaploid triticale

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Induction medium osmolality improves microspore embryogenesis in wheat and triticale

Changes in gene expression patterns associated with microspore embryogenesis in hexaploid triticale (×Triticosecale Wittm.)

Translocation of cell-penetrating peptides and delivery of their cargoes in triticale microspores

Organelle antioxidants improve microspore embryogenesis in wheat and triticale

DsDNA and protein co-delivery in triticale microspores

Gametic embryogenesis through isolated microspore culture in Corylus avellana L.

Dimethyl tyrosine conjugated peptide prevents oxidative damage and death of triticale and wheat microspores