NDLI: Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Content Provider	Springer Nature Link
Author	Żur, Iwona Dubas, Ewa Krzewska, Monika Jawiak, Franciszek Hura, Katarzyna Pociecha, Ewa Bączek Kwinta, Renata Płażek, Agnieszka
Copyright Year	2014
Abstract	To verify the hypothesis that cell redox status regulates the process of microspore embryogenesis (ME), reactive oxygen species (ROS) generation and the activity of enzymatic and non-enzymatic antioxidants were analyzed in eight doubled haploid lines of triticale with significant differences in embryogenic potential. The analyses were performed in anthers excised from freshly cut tillers (control) and from low temperature (LT) pre-treated tillers (3 weeks at 4 °C) in which ME has been initiated. Significant associations between ME effectiveness and the variables studied were found. In control cultures, high superoxide dismutase (SOD) activity appeared crucial for microspore viability. On the other hand, positive though non-linear correlation between ME effectiveness and H$_{2}$O$_{2}$ generation, and negative correlation with catalase (CAT) activity suggest that some threshold level of H$_{2}$O$_{2}$ is important for successful ME initiation. LT tillers pre-treatment significantly increased H$_{2}$O$_{2}$ accumulation, which had a negative effect on ME effectiveness. However, even high level of H$_{2}$O$_{2}$ did not endanger cell viability as long as the cells exhibited high activity of ROS-decomposing enzymes (SOD, CAT and POX). The ability to sustain antioxidative enzyme activity under cold stress in the dark was another important requirement for high effectiveness of ME, allowing for the generation of the signal initiating microspore reprogramming and simultaneously protecting the cells from the toxic effects of ROS production. The role of antioxidative enzymes cannot be replaced even by high activity of non-enzymatic antioxidants. In conclusion, genetically controlled but environmentally modified cell tolerance to oxidative stress seems to play an important role in triticale ME.
Starting Page	79
Ending Page	94
Page Count	16
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	119
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-06-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Androgenesis Antioxidant activity Cold stress Oxidative stress Reactive oxygen species Triticale Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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